| http://purl.uniprot.org/citations/11085990 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11085990 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11085990 | http://www.w3.org/2000/01/rdf-schema#comment | "The growing public health problem of infections caused by multiresistant Gram-positive bacteria, in particular Staphylococcus aureus, prompted us to screen human epithelia for endogenous S. aureus-killing factors. A novel 5-kDa, nonhemolytic antimicrobial peptide (human beta-defensin-3, hBD-3) was isolated from human lesional psoriatic scales and cloned from keratinocytes. hBD-3 demonstrated a salt-insensitive broad spectrum of potent antimicrobial activity against many potentially pathogenic microbes including multiresistant S. aureus and vancomycin-resistant Enterococcus faecium. Ultrastructural analyses of hBD-3-treated S. aureus revealed signs of cell wall perforation. Recombinant hBD-3 (expressed as a His-Tag-fusion protein in Escherichia coli) and chemically synthesized hBD-3 were indistinguishable from naturally occurring peptide with respect to their antimicrobial activity and biochemical properties. Investigation of different tissues revealed skin and tonsils to be major hBD-3 mRNA-expressing tissues. Molecular cloning and biochemical analyses of antimicrobial peptides in cell culture supernatants revealed keratinocytes and airway epithelial cells as cellular sources of hBD-3. Tumor necrosis factor alpha and contact with bacteria were found to induce hBD-3 mRNA expression. hBD-3 therefore might be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung, such as cystic fibrosis."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m008557200"xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m008557200"xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/author | "Harder J."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/author | "Harder J."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/author | "Bartels J."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/author | "Bartels J."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/author | "Christophers E."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/author | "Christophers E."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/author | "Schroeder J.-M."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/author | "Schroeder J.-M."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/pages | "5707-5713"xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/pages | "5707-5713"xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/title | "Isolation and characterization of human beta-defensin-3, a novel human inducible peptide antibiotic."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/title | "Isolation and characterization of human beta-defensin-3, a novel human inducible peptide antibiotic."xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11085990 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11085990 |
| http://purl.uniprot.org/citations/11085990 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11085990 |