| http://purl.uniprot.org/citations/11101529 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11101529 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11101529 | http://www.w3.org/2000/01/rdf-schema#comment | "Recently, we identified proteins that co-purify with the human spliceosome using mass spectrometry. One of the identified proteins, CDC5L, corresponds to the human homologue of the Schizosaccharomyces pombe CDC5(+) gene product. Here we show that CDC5L is part of a larger multiprotein complex in HeLa nuclear extract that incorporates into the spliceosome in an ATP-dependent step. We also show that this complex is required for the second catalytic step of pre-mRNA splicing. Immunodepletion of the CDC5L complex from HeLa nuclear extract inhibits the formation of pre-mRNA splicing products in vitro but does not prevent spliceosome assembly. The first catalytic step of pre-mRNA splicing is less affected by immunodepleting the complex. The purified CDC5L complex in HeLa nuclear extract restores pre-mRNA splicing activity when added to extracts that have been immunodepleted using anti-CDC5L antibodies. Using mass spectrometry and database searches, the major protein components of the CDC5L complex have been identified. This work reports a first purification and characterization of a functional, human non-snRNA spliceosome subunit containing CDC5L and at least five additional protein factors."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/19.23.6569"xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/19.23.6569"xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Kuster B."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Kuster B."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Mann M."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Mann M."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Lamond A.I."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Lamond A.I."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Ajuh P."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Ajuh P."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Panov K."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Panov K."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Zomerdijk J.C.B.M."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/author | "Zomerdijk J.C.B.M."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/pages | "6569-6581"xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/pages | "6569-6581"xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/title | "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry."xsd:string |
| http://purl.uniprot.org/citations/11101529 | http://purl.uniprot.org/core/title | "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry."xsd:string |