| http://purl.uniprot.org/citations/11102511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11102511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11102511 | http://www.w3.org/2000/01/rdf-schema#comment | "Sorting nexin (SNX) 1 and SNX2 are mammalian orthologs of Vps5p, a yeast protein that is a subunit of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. We report the cloning and characterization of human orthologs of three additional components of the complex: Vps26p, Vps29p, and Vps35p. The close structural similarity between the yeast and human proteins suggests a similarity in function. We used both yeast two-hybrid assays and expression in mammalian cells to define the binding interactions among these proteins. The data suggest a model in which hVps35 serves as the core of a multimeric complex by binding directly to hVps26, hVps29, and SNX1. Deletional analyses of hVps35 demonstrate that amino acid residues 1-53 and 307-796 of hVps35 bind to the coiled coil-containing domain of SNX1. In contrast, hVps26 binds to amino acid residues 1-172 of hVps35, whereas hVps29 binds to amino acid residues 307-796 of hVps35. Furthermore, hVps35, hVps29, and hVps26 have been found in membrane-associated and cytosolic compartments. Gel filtration chromatography of COS7 cell cytosol showed that both recombinant and endogenous hVps35, hVps29, and hVps26 coelute as a large complex ( approximately 220-440 kDa). In the absence of hVps35, neither hVps26 nor hVps29 is found in the large complex. These data provide the first insights into the binding interactions among subunits of a putative mammalian retromer complex."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.11.12.4105"xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.11.12.4105"xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "Barr V.A."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "Barr V.A."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "Taylor S.I."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "Taylor S.I."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "de la Luz Sierra M."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "de la Luz Sierra M."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "Bafford R."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "Bafford R."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "Lesniak M.A."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "Lesniak M.A."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "Renfrew Haft C."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/author | "Renfrew Haft C."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/pages | "4105-4116"xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/pages | "4105-4116"xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/title | "Human orthologs of yeast vacuolar protein sorting proteins Vps26, 29, and 35: assembly into multimeric complexes."xsd:string |
| http://purl.uniprot.org/citations/11102511 | http://purl.uniprot.org/core/title | "Human orthologs of yeast vacuolar protein sorting proteins Vps26, 29, and 35: assembly into multimeric complexes."xsd:string |