| http://purl.uniprot.org/citations/11102533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11102533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11102533 | http://www.w3.org/2000/01/rdf-schema#comment | "In yeast, the Ypt1 GTPase is required for ER-to-cis-Golgi and cis-to-medial-Golgi protein transport, while Ypt31/32 are a functional pair of GTPases essential for exit from the trans-Golgi. We have previously identified a Ypt1 guanine nucleotide exchange factor (GEF) activity and characterized it as a large membrane-associated protein complex that localizes to the Golgi and can be extracted from the membrane by salt, but not by detergent. TRAPP is a large protein complex that is required for ER-to-Golgi transport and that has properties similar to those of Ypt1 GEF. Here we show that TRAPP has Ypt1 GEF activity. GST-tagged Bet3p or Bet5p, two of the TRAPP subunits, were expressed in yeast cells and were precipitated by glutathione-agarose (GA) beads. The resulting precipitates can stimulate both GDP release and GTP uptake by Ypt1p. The majority of the Ypt1 GEF activity associated with the GST-Bet3p precipitate has an apparent molecular weight of > 670 kDa, indicating that the GEF activity resides in the TRAPP complex. Surprisingly, TRAPP can also stimulate nucleotide exchange on the Ypt31/32 GTPases, but not on Sec4p, a Ypt-family GTPase required for the last step of the exocytic pathway. Like the previously characterized Ypt1 GEF, the TRAPP Ypt1-GEF activity can be inhibited by the nucleotide-free Ypt1-D124N mutant protein. This mutant protein also inhibits the Ypt32 GEF activity of TRAPP. Coprecipitation and overexpression studies suggest that TRAPP can act as a GEF for Ypt1 and Ypt31/32 in vivo. These data suggest the exciting possibility that a GEF complex common to Ypt1 and Ypt31/32 might coordinate the function of these GTPases in entry into and exit from the Golgi."xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.11.12.4403"xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.11.12.4403"xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/author | "Jones S."xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/author | "Jones S."xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/author | "Liu F."xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/author | "Liu F."xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/author | "Newman C."xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/author | "Newman C."xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/author | "Segev N."xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/author | "Segev N."xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/pages | "4403-4411"xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/pages | "4403-4411"xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/title | "The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32."xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/title | "The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32."xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/11102533 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11102533 |
| http://purl.uniprot.org/citations/11102533 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11102533 |