| http://purl.uniprot.org/citations/11113139 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11113139 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11113139 | http://www.w3.org/2000/01/rdf-schema#comment | "CD4 recruitment to T cell receptor (TCR)-peptide-major histocompatibility class II complexes is required for stabilization of low affinity antigen recognition by T lymphocytes. The cytoplasmic portion of CD4 is thought to amplify TCR-initiated signal transduction via its association with the protein tyrosine kinase p56(lck). Here we describe a novel functional determinant in the cytosolic tail of CD4 that inhibits TCR-induced T cell activation. Deletion of two conserved hydrophobic amino acids from the CD4 carboxyl terminus resulted in a pronounced enhancement of CD4-mediated T cell costimulation. This effect was observed in the presence or absence of p56(lck), implying involvement of alternative cytosolic ligands of CD4. A two-hybrid screen with the intracellular portion of CD4 identified a previously unknown 33-kDa protein, ACP33 (acidic cluster protein 33), as a novel intracellular binding partner of CD4. Since interaction with ACP33 is abolished by deletion of the hydrophobic CD4 C-terminal amino acids mediating repression of T cell activation, we propose that ACP33 modulates the stimulatory activity of CD4. Furthermore, we demonstrate that interaction with CD4 is mediated by the noncatalytic alpha/beta hydrolase fold domain of ACP33. This suggests a previously unrecognized function for alpha/beta hydrolase fold domains as a peptide binding module mediating protein-protein interactions."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m009270200"xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m009270200"xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/author | "Kremmer E."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/author | "Kremmer E."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/author | "Kolanus W."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/author | "Kolanus W."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/author | "Sirim P."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/author | "Sirim P."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/author | "Zeitlmann L."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/author | "Zeitlmann L."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/pages | "9123-9132"xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/pages | "9123-9132"xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/title | "Cloning of ACP33 as a novel intracellular ligand of CD4."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/title | "Cloning of ACP33 as a novel intracellular ligand of CD4."xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11113139 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11113139 |
| http://purl.uniprot.org/citations/11113139 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11113139 |