| http://purl.uniprot.org/citations/11118204 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11118204 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11118204 | http://www.w3.org/2000/01/rdf-schema#comment | "Escherichia coli K-12, the most widely used laboratory bacterium, does not secrete proteins into the extracellular medium under standard growth conditions, despite possessing chromosomal genes encoding a putative type II secretion machinery (secreton). We show that in wild-type E.coli K-12, divergent transcription of the two operons in the main chromosomal gsp locus, encoding the majority of the secreton components, is silenced by the nucleoid-structuring protein H-NS. In mutants lacking H-NS, the secreton genes cloned on a moderate-copy-number plasmid are expressed and promote efficient secretion of the endogenous, co-regulated endochitinase ChiA. This is the first time that secretion of an endogenous extracellular protein has been demonstrated in E.coli K-12."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/19.24.6697"xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/19.24.6697"xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/author | "Belin D."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/author | "Belin D."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/author | "Pugsley A.P."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/author | "Pugsley A.P."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/author | "Badaut C."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/author | "Badaut C."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/author | "Francetic O."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/author | "Francetic O."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/pages | "6697-6703"xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/pages | "6697-6703"xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/title | "Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/title | "Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion."xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/11118204 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11118204 |
| http://purl.uniprot.org/citations/11118204 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11118204 |