| http://purl.uniprot.org/citations/11134424 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11134424 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11134424 | http://www.w3.org/2000/01/rdf-schema#comment | "A new isogene for acyl-(acyl-carrier-protein):glycerol-3-phosphate acyltransferase (GPAT; EC 2.3.1.15) in squash has been cloned and the gene product was identified as oleate-selective GPAT. Using PCR primers that could hybridise with exons for a previously cloned squash GPAT, we obtained two PCR products of different size: one coded for a previously cloned squash GPAT corresponding to non-selective isoforms AT2 and AT3, and the other for a new isozyme, probably the oleate-selective isoform AT1. Full-length amino acid sequences of respective isozymes were deduced from the nucleotide sequences of genomic genes and cDNAs, which were cloned by a series of PCR-based methods. Thus, we designated the new gene CmATS1;1 and the other one CmATS1;2. Genome blot analysis revealed that the squash genome contained the two isogenes at non-allelic loci. AT1-active fractions were partially purified, and three polypeptide bands were identified as being AT1 polypeptides, which exhibited relative molecular masses of 39.5-40.5 kDa, pI values of 6.75-7.15, and oleate selectivity over palmitate. Partial amino-terminal sequences obtained from two of these bands verified that the new isogene codes for AT1 polypeptides."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.org/dc/terms/identifier | "doi:10.1093/pcp/pcd072"xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.org/dc/terms/identifier | "doi:10.1093/pcp/pcd072"xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/author | "Enju A."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/author | "Enju A."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/author | "Nakamura M."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/author | "Nakamura M."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/author | "Nishida I."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/author | "Nishida I."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/author | "Sugiura M."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/author | "Sugiura M."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/date | "2000"xsd:gYear |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/name | "Plant Cell Physiol."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/name | "Plant Cell Physiol."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/pages | "1381-1391"xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/pages | "1381-1391"xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/title | "A second gene for acyl-(acyl-carrier-protein): glycerol-3-phosphate acyltransferase in squash, Cucurbita moschata cv. Shirogikuza(*), codes for an oleate-selective isozyme: molecular cloning and protein purification studies."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/title | "A second gene for acyl-(acyl-carrier-protein): glycerol-3-phosphate acyltransferase in squash, Cucurbita moschata cv. Shirogikuza(*), codes for an oleate-selective isozyme: molecular cloning and protein purification studies."xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/volume | "41"xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://purl.uniprot.org/core/volume | "41"xsd:string |
| http://purl.uniprot.org/citations/11134424 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11134424 |
| http://purl.uniprot.org/citations/11134424 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11134424 |