| http://purl.uniprot.org/citations/11139622 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11139622 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11139622 | http://www.w3.org/2000/01/rdf-schema#comment | "Gamma-crystallin genes are specifically expressed in the eye lens. Their promoters constitute excellent models to analyse tissue-specific gene expression. We investigated murine CRYGE/f promoters of different length in lens epithelial cell lines. The most active fragment extends from position -219 to +37. Computer analysis predicts homeodomain and paired-domain binding sites for all rodent CRYGD/e/f core promoters. As examples, we analysed the effects of Prox1 and Six3, which are considered important transcription factors involved in lens development. Because of endogenous Prox1 expression in N/N1003A cells, a weak stimulation of CRYGE/f promoter activity was found for PROX1. In contrast, PROX1 stimulated the CRYGF promoter 10-fold in CD5A cells without endogenous PROX1. In both cell lines Six3 repressed the CRYGF promoter to 10% of its basal activity. Our cell transfection experiments indicated that CRYG expression increases as Six3 expression decreases. Prox1 and Six3 act antagonistically on regulation of the CRYGD/e/f promoters. Functional assays using randomly mutated gammaF-crystallin promoter fragments define a Six3-responsive element between -101 and -123 and a Prox1-responsive element between -151 and -174. Since Prox1 and Six3 are present at the beginning of lens development, expression of CRYGD/e/f is predicted to remain low at this time. It increases as Six3 expression decreases during ongoing lens development."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/29.2.515"xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/29.2.515"xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Prescott A."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Prescott A."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Quinlan R.A."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Quinlan R.A."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Graw J."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Graw J."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Tomarev S."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Tomarev S."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Krausz E."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Krausz E."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Lengler J."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/author | "Lengler J."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/pages | "515-526"xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/pages | "515-526"xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/title | "Antagonistic action of Six3 and Prox1 at the gamma-crystallin promoter."xsd:string |
| http://purl.uniprot.org/citations/11139622 | http://purl.uniprot.org/core/title | "Antagonistic action of Six3 and Prox1 at the gamma-crystallin promoter."xsd:string |