RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/11158529http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11158529http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11158529http://www.w3.org/2000/01/rdf-schema#comment"Full-length cDNAs are essential for functional analysis of plant genes. Using the biotinylated CAP trapper method, we constructed full-length Arabidopsis cDNA libraries from plants in different conditions, such as drought-treated, cold-treated, or unstressed plants, and at various developmental stages from germination to mature seed. We prepared a cDNA microarray using approximately 1300 full-length Arabidopsis cDNAs to identify drought- and cold-inducible genes and target genes of DREB1A/CBF3, a transcription factor that controls stress-inducible gene expression. In total, 44 and 19 cDNAs for drought- and cold-inducible genes, respectively, were isolated, 30 and 10 of which were novel stress-inducible genes that have not been reported as drought- or cold-inducible genes previously. Twelve stress-inducible genes were identified as target stress-inducible genes of DREB1A, and six of them were novel. On the basis of RNA gel blot and microarray analyses, the six genes were identified as novel drought- and cold-inducible genes that are controlled by DREB1A. Eleven DREB1A target genes whose genomic sequences have been registered in the GenBank database contained the dehydration-responsive element (DRE) or DRE-related CCGAC core motif in their promoter regions. These results show that our full-length cDNA microarray is a useful material with which to analyze the expression pattern of Arabidopsis genes under drought and cold stresses, to identify target genes of stress-related transcription factors, and to identify potential cis-acting DNA elements by combining the expression data with the genomic sequence data."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.org/dc/terms/identifier"doi:10.1105/tpc.13.1.61"xsd:string
http://purl.uniprot.org/citations/11158529http://purl.org/dc/terms/identifier"doi:10.1105/tpc.13.1.61"xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Abe H."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Abe H."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Carninci P."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Carninci P."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Hayashizaki Y."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Hayashizaki Y."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Kasuga M."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Kasuga M."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Narusaka M."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Narusaka M."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Seki M."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Seki M."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Shinozaki K."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Shinozaki K."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Yamaguchi-Shinozaki K."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/author"Yamaguchi-Shinozaki K."xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/name"Plant Cell"xsd:string
http://purl.uniprot.org/citations/11158529http://purl.uniprot.org/core/name"Plant Cell"xsd:string