| http://purl.uniprot.org/citations/11168362 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11168362 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11168362 | http://www.w3.org/2000/01/rdf-schema#comment | "The dust mite Lepidoglyphus destructor is a common species in Europe and a major cause of dust mite allergy in rural surroundings, but it also contributes to dust mite allergy in urban areas. One major allergen, Lep d 2, has been expressed as a recombinant protein and evaluated both in vivo and in vitro and shown to detect 60% or more of L. destructor-sensitized subjects. Additional recombinant allergens are needed to obtain a reliable diagnostic tool for L. destructor allergy. The aim of this study was to clone and express new allergens from L. destructor and determine their recognition frequency among sensitized individuals. A phage display cDNA expression library was constructed and screened with sera from L. destructor-sensitized individuals. The cDNAs encoding the allergens were cloned into the pET17b vector and subsequently expressed in Escherichia coli as C-terminal His6-tagged proteins. Immunoblotting of the recombinant proteins was performed using sera from 45 subjects allergic to L. destructor. Three new allergens from L. destructor, Ld 5 (originating from a partial Lep d 5 clone), Lep d 7 and Lep d 13, were identified and recognized by 4/45 (9%), 28/45 (62%) and 6/45 (13%) sera from L. destructor-sensitized subjects, respectively."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1432-1327.2001.01879.x"xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1432-1327.2001.01879.x"xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Whitley P."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Whitley P."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Huecas S."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Huecas S."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Crameri R."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Crameri R."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Gafvelin G."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Gafvelin G."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "van Hage-Hamsten M."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "van Hage-Hamsten M."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Appenzeller U."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Appenzeller U."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Eriksson T.L.J."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Eriksson T.L.J."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Rasool O."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/author | "Rasool O."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/11168362 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |