| http://purl.uniprot.org/citations/11179219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11179219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11179219 | http://www.w3.org/2000/01/rdf-schema#comment | "Three classes of mammalian phosphoinositide-specific phospholipase C (PLC) have been characterized, PLCbeta, PLCgamma and PLCdelta, that are differentially regulated by heterotrimeric G-proteins, tyrosine kinases and calcium. Here we describe a fourth class, PLCepsilon, that in addition to conserved PLC domains, contains a GTP exchange factor (GRF CDC25) domain and two C-terminal Ras-binding (RA) domains, RA1 and RA2. The RA2 domain binds H-Ras in a GTP-dependent manner, comparable with the Ras-binding domain of Raf-1; however, the RA1 domain binds H-Ras with a low affinity in a GTP-independent manner. While G(alpha)q, Gbetagamma or, surprisingly, H-Ras do not activate recombinant purified protein in vitro, constitutively active Q61L H-Ras stimulates PLC(epsilon) co-expressed in COS-7 cells in parallel with Ras binding. Deletion of either the RA1 or RA2 domain inhibits this activation. Site-directed mutagenesis of the RA2 domain or Ras demonstrates a conserved Ras-effector interaction and a unique profile of activation by Ras effector domain mutants. These studies identify a novel fourth class of mammalian PLC that is directly regulated by Ras and links two critical signaling pathways."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/20.4.743"xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/20.4.743"xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/author | "Smrcka A.V."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/author | "Smrcka A.V."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/author | "Kelley G.G."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/author | "Kelley G.G."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/author | "Ondrako J.M."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/author | "Ondrako J.M."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/author | "Reks S.E."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/author | "Reks S.E."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/pages | "743-754"xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/pages | "743-754"xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/title | "Phospholipase C(epsilon): a novel Ras effector."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/title | "Phospholipase C(epsilon): a novel Ras effector."xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/11179219 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11179219 |
| http://purl.uniprot.org/citations/11179219 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11179219 |