Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/11230971http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11230971http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11230971http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Adrenomedullin (AM) is a potent vasodilator peptide. Plasma AM concentration is increased in patients with various heart diseases, and both myocytes (MCs) and non-myocytes (NMCs) secrete AM and express its receptors. These facts suggest that cardiac cells possess an autocrine/paracrine capability mediated by AM.

Methods

MCs and NMCs were prepared from cardiac ventricles of neonatal rats. AM and endothelin-1 concentrations were measured by radioimmunoassays, and interleukin-6 level by a specific bioassay. Total nitrite/nitrate contents were measured with a fluorescence assay kit.

Results

A basal secretion rate of AM from NMCs was 2.8-fold higher than that from MCs. Interleukin-1beta, tumor necrosis factor-alpha and lipopolysaccharide stimulated AM secretion from NMCs but not from MCs. AM stimulated interleukin-6 production in the presence of these cytokines or lipopolysaccharide, which was more prominent in NMCs. In the presence of interleukin-1beta, AM augmented nitric oxide synthesis 2.7-fold in NMCs, but slightly in MCs. NMCs secreted endothelin-1 at a rate nine times higher than MCs, and AM inhibited endothelin-1 secretion from NMCs.

Conclusion

This in vitro study suggests that AM in the heart is mainly produced in NMCs and exerts its effects through NMCs, especially under inflammatory conditions."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.org/dc/terms/identifier"doi:10.1016/s0008-6363(00)00291-1"xsd:string
http://purl.uniprot.org/citations/11230971http://purl.org/dc/terms/identifier"doi:10.1016/s0008-6363(00)00291-1"xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Tanaka A."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Tanaka A."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Kangawa K."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Kangawa K."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Minamino N."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Minamino N."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Dohi K."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Dohi K."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Katafuchi T."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Katafuchi T."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Kikumoto K."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Kikumoto K."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Tomoda Y."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Tomoda Y."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Isumi Y."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/author"Isumi Y."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/name"Cardiovasc. Res."xsd:string
http://purl.uniprot.org/citations/11230971http://purl.uniprot.org/core/name"Cardiovasc. Res."xsd:string
▶