| http://purl.uniprot.org/citations/11238902 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11238902 | http://www.w3.org/2000/01/rdf-schema#comment | "In Saccharomyces cerevisiae, the AP endonucleases encoded by the APN1 and APN2 genes provide alternate pathways for the removal of abasic sites. Oxidative DNA-damaging agents, such as H(2)O(2), produce DNA strand breaks which contain 3'-phosphate or 3'-phosphoglycolate termini. Such 3' termini are inhibitory to synthesis by DNA polymerases. Here, we show that purified yeast Apn2 protein contains 3'-phosphodiesterase and 3'-->5' exonuclease activities, and mutation of the active-site residue Glu59 to Ala in Apn2 inactivates both these activities. Consistent with these biochemical observations, genetic studies indicate the involvement of APN2 in the repair of H(2)O(2)-induced DNA damage in a pathway alternate to APN1, and the Ala59 mutation inactivates this function of Apn2. From these results, we conclude that the ability of Apn2 to remove 3'-end groups from DNA is paramount for the repair of strand breaks arising from the reaction of DNA with reactive oxygen species."xsd:string |
| http://purl.uniprot.org/citations/11238902 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.21.5.1656-1661.2001"xsd:string |
| http://purl.uniprot.org/citations/11238902 | http://purl.uniprot.org/core/author | "Haracska L."xsd:string |
| http://purl.uniprot.org/citations/11238902 | http://purl.uniprot.org/core/author | "Prakash L."xsd:string |
| http://purl.uniprot.org/citations/11238902 | http://purl.uniprot.org/core/author | "Prakash S."xsd:string |
| http://purl.uniprot.org/citations/11238902 | http://purl.uniprot.org/core/author | "Unk I."xsd:string |
| http://purl.uniprot.org/citations/11238902 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11238902 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/11238902 | http://purl.uniprot.org/core/pages | "1656-1661"xsd:string |
| http://purl.uniprot.org/citations/11238902 | http://purl.uniprot.org/core/title | "3'-phosphodiesterase and 3'-->5' exonuclease activities of yeast Apn2 protein and requirement of these activities for repair of oxidative DNA damage."xsd:string |
| http://purl.uniprot.org/citations/11238902 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/11238902 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11238902 |
| http://purl.uniprot.org/citations/11238902 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11238902 |
| http://purl.uniprot.org/uniprot/P38207#attribution-8DFF5FCBE2519BCE0F48E3F0192BAEF3 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11238902 |
| http://purl.uniprot.org/uniprot/#_P38207-mappedCitation-11238902 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11238902 |
| http://purl.uniprot.org/uniprot/P38207 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11238902 |