| http://purl.uniprot.org/citations/11240126 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11240126 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11240126 | http://www.w3.org/2000/01/rdf-schema#comment | "Nck-2 is a newly identified adapter protein comprising three N-terminal SH3 domains and one C-terminal SH2 domain. We have identified in a yeast two-hybrid screen DOCK180, a signaling protein implicated in the regulation of membrane ruffling and migration, as a binding protein for Nck-2. Surface plasmon resonance analyses reveal that the second and the third SH3 domains interact with the C-terminal region of DOCK180. The interactions mediated by the individual SH3 domains, however, are much weaker than that of the full length Nck-2. Furthermore, a point mutation that inactivates the second or the third SH3 domain dramatically reduced the interaction of Nck-2 with DOCK180, suggesting that both SH3 domains contribute to the DOCK180 binding. A major Nck-2 binding site, which is recognized primarily by the third SH3 domain, has been mapped to residues 1819-1836 of DOCK180. Two additional, albeit much weaker, Nck-2 SH3 binding sites are located to DOCK180 residues 1793-1810 and 1835-1852 respectively. Consistent with the mutational studies, kinetic analyses by surface plasmon resonance suggest that two binding events with equilibrium dissociation constants of 4.15+/-1.9x10(-7) M and 3.24+/-1.9x10(-9) M mediate the binding of GST-Nck-2 to GST fusion protein containing the C-terminal region of DOCK180. These studies identify a novel interaction between Nck-2 and DOCK180. Furthermore, they provide a detailed analysis of a protein complex formation mediated by multiple SH3 domains revealing that tandem SH3 domains significantly enhance the weak interactions mediated by each individual SH3 domain."xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0014-5793(01)02195-0"xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0014-5793(01)02195-0"xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/author | "Tu Y."xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/author | "Tu Y."xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/author | "Wu C."xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/author | "Wu C."xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/author | "Kucik D.F."xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/author | "Kucik D.F."xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/name | "FEBS Lett."xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/name | "FEBS Lett."xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/pages | "193-199"xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/pages | "193-199"xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/title | "Identification and kinetic analysis of the interaction between Nck-2 and DOCK180."xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/title | "Identification and kinetic analysis of the interaction between Nck-2 and DOCK180."xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/volume | "491"xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://purl.uniprot.org/core/volume | "491"xsd:string |
| http://purl.uniprot.org/citations/11240126 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11240126 |
| http://purl.uniprot.org/citations/11240126 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11240126 |
| http://purl.uniprot.org/citations/11240126 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11240126 |
| http://purl.uniprot.org/citations/11240126 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11240126 |