| http://purl.uniprot.org/citations/11250908 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11250908 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11250908 | http://www.w3.org/2000/01/rdf-schema#comment | "GCN2 stimulates translation of GCN4 mRNA in amino acid-starved cells by phosphorylating translation initiation factor 2. GCN2 is activated by binding of uncharged tRNA to a domain related to histidyl-tRNA synthetase (HisRS). The HisRS-like region contains two dimerization domains (HisRS-N and HisRS-C) required for GCN2 function in vivo but dispensable for dimerization by full-length GCN2. Residues corresponding to amino acids at the dimer interface of Escherichia coli HisRS were required for dimerization of recombinant HisRS-N and for tRNA binding by full-length GCN2, suggesting that HisRS-N dimerization promotes tRNA binding and kinase activation. HisRS-N also interacted with the protein kinase (PK) domain, and a deletion impairing this interaction destroyed GCN2 function without reducing tRNA binding; thus, HisRS-N-PK interaction appears to stimulate PK function. The C-terminal domain of GCN2 (C-term) interacted with the PK domain in a manner disrupted by an activating PK mutation (E803V). These results suggest that the C-term is an autoinhibitory domain, counteracted by tRNA binding. We conclude that multiple domain interactions, positive and negative, mediate the activation of GCN2 by uncharged tRNA."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/20.6.1425"xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/20.6.1425"xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/author | "Dong J."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/author | "Dong J."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/author | "Hu C."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/author | "Hu C."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/author | "Qiu H."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/author | "Qiu H."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/author | "Francklyn C.S."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/author | "Francklyn C.S."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/author | "Hinnebusch A.G."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/author | "Hinnebusch A.G."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/pages | "1425-1438"xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/pages | "1425-1438"xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/title | "The tRNA-binding moiety in GCN2 contains a dimerization domain that interacts with the kinase domain and is required for tRNA binding and kinase activation."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/title | "The tRNA-binding moiety in GCN2 contains a dimerization domain that interacts with the kinase domain and is required for tRNA binding and kinase activation."xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/11250908 | http://purl.uniprot.org/core/volume | "20"xsd:string |