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http://purl.uniprot.org/citations/11292825http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11292825http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11292825http://www.w3.org/2000/01/rdf-schema#comment"Inactivation of the visual G protein transducin, during recovery from photoexcitation, is regulated by RGS9-1, a GTPase-accelerating protein of the ubiquitous RGS protein family. Incubation of dark-adapted bovine rod outer segments with [gamma-(32)P]ATP led to RGS9-1 phosphorylation by an endogenous kinase in rod outer segment membranes, with an average stoichiometry of 0.2-0.45 mol of phosphates/mol of RGS9-1. Mass spectrometry revealed a single major site of phosphorylation, Ser(475). The kinase responsible catalyzed robust phosphorylation of recombinant RGS9-1 and not of an S475A mutant. A synthetic peptide corresponding to the region surrounding Ser(475) was also phosphorylated, and a similar peptide with the S475A substitution inhibited RGS9-1 phosphorylation. The RGS9-1 kinase is a peripheral membrane protein that co-purifies with rhodopsin in sucrose gradients and can be extracted in buffers of high ionic strength. It is not inhibited or activated significantly by a panel of inhibitors or activators of protein kinase A, protein kinase G, rhodopsin kinase, CaM kinase II, casein kinase II, or cyclin-dependent kinase 5, at concentrations 50 or more times higher than their reported IC(50) or K(i) values. It was inhibited by the protein kinase C inhibitor bisindolylmaleimide I and by lowering Ca(2+) to nanomolar levels with EGTA; however, it was not stimulated by the addition of phorbol ester, under conditions that significantly enhanced rhodopsin phosphorylation. A monoclonal antibody specific for the Ser(475)-phosphorylated form of RGS9-1 recognized RGS9-1 in immunoblots of dark-adapted mouse retina. Retinas from light-adapted mice had much lower levels of RGS9-1 phosphorylation. Thus, RGS9-1 is phosphorylated on Ser(475) in vivo, and the phosphorylation level is regulated by light and by [Ca(2+)], suggesting the importance of the modification in light adaptation."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m011539200"xsd:string
http://purl.uniprot.org/citations/11292825http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m011539200"xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/author"Hu G."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/author"Hu G."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/author"Palczewski K."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/author"Palczewski K."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/author"Wensel T.G."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/author"Wensel T.G."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/author"Cowan C.W."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/author"Cowan C.W."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/author"Jang G.F."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/author"Jang G.F."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/pages"22287-22295"xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/pages"22287-22295"xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/title"Phosphorylation of RGS9-1 by an endogenous protein kinase in rod outer segments."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/title"Phosphorylation of RGS9-1 by an endogenous protein kinase in rod outer segments."xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/volume"276"xsd:string
http://purl.uniprot.org/citations/11292825http://purl.uniprot.org/core/volume"276"xsd:string
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