| http://purl.uniprot.org/citations/11298758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11298758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11298758 | http://www.w3.org/2000/01/rdf-schema#comment | "Activator protein-2 (AP-2) has been implicated as a transactivator of the human and ovine placental lactogen (oPL) genes. Transcriptional enhancement through an AP-2 cis-acting element has been described for other genes expressed in the placenta, but the AP-2 isoform enhancing expression is species dependent. Transactivation of the oPL minimal promoter (-124 bp to +16 bp) by AP-2 was confirmed by mutational analysis in transiently transfected human choriocarcinoma cells (BeWo). AP-2alpha was localized in ovine chorionic epithelial cells by immunohistochemistry and a 3-kb transcript was identified by Northern hybridization. Four nearly full-length AP-2 cDNAs were isolated from an ovine placenta cDNA library. Nucleotide sequencing these cDNAs revealed that the AP-2 mRNA expressed in the ovine placenta shares identity with human AP-2alpha, but variations in the predicted N-terminus were observed, and three unique AP-2alpha splice-variants were identified. Expression of AP-2alpha variants in HepG2 cells, devoid of endogenous AP-2, indicates that enhancement through the AP-2 element in the oPL gene minimal promoter was variant dependent. RNA transcripts for all of the ovine AP-2alpha splice-variants were confirmed in ovine placenta by RT-PCR, and homologs for two variants were found in human placenta. However, only one AP-2alpha transcript, which shares identity to Xenopus AP-2alpha, was expressed in BeWo cells. Immunoblot analysis confirmed AP-2alpha variants in ovine chorionic binucleate cell nuclear extracts, one of which migrates similar to the AP-2alpha variant identified in BeWo cell nuclear extracts. These data indicate the presence of new mammalian AP-2alpha splice-variants that augment transactivation of the oPL gene in ovine chorionic binucleate cells."xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1432-1327.2001.02124.x"xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1432-1327.2001.02124.x"xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/author | "Anthony R.V."xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/author | "Anthony R.V."xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/author | "Limesand S.W."xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/author | "Limesand S.W."xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/pages | "2390-2401"xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/pages | "2390-2401"xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/title | "Novel activator protein-2alpha splice-variants function as transactivators of the ovine placental lactogen gene."xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/title | "Novel activator protein-2alpha splice-variants function as transactivators of the ovine placental lactogen gene."xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/volume | "268"xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://purl.uniprot.org/core/volume | "268"xsd:string |
| http://purl.uniprot.org/citations/11298758 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11298758 |
| http://purl.uniprot.org/citations/11298758 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11298758 |
| http://purl.uniprot.org/citations/11298758 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11298758 |
| http://purl.uniprot.org/citations/11298758 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11298758 |
| http://purl.uniprot.org/uniprot/Q9N0N3 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/11298758 |
| http://purl.uniprot.org/uniprot/Q9N0N3#attribution-6DDDEE02089A702FFF6258D0A489BCEE | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11298758 |