Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/11318877http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11318877http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11318877http://www.w3.org/2000/01/rdf-schema#comment"

Background

Development of the pancreas and the nervous tissues is regulated by common transcription factors. A basic helix-loop-helix protein, p48 of pancreas transcription factor 1 (PTF1), is essential for differentiation of the exocrine acinar cells.

Results

We isolated PTF1 p48 from 9.5-day mouse embryos as a binding protein of RBP-Jkappa, a mediator of Notch signalling. p48 bound to RBP-Jkappa more strongly than and in a distinct way from Notch1. In 9.5-12.5 day embryos, p48 was expressed in the dorsal part of the neural tube as well as in the pancreatic buds. Two lines of evidence suggested functions of p48 in neurogenesis: (i) expression of p48 was induced in P19 cells when they committed to neural fate upon retinoic acid treatment, and (ii) p48 over-expressed in Xenopus embryos repressed the development of neuronal precursors. p48 inhibited the MASH1-activated transcription from the E-box, while p48 stimulated transcription from the PTF1 motif synergistically with E47. The p48/E47-activated transcription from the PTF1 motif was stimulated further by RBP-Jkappa and RBP-Jkappa derivatives that mimicked the active RBP-Jkappa/Notch complex.

Conclusions

In developing embryos, p48 is expressed in both the nervous system and the pancreas. p48 inhibits neuronal differentiation. We propose possible mechanisms for this inhibition."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-2443.2001.00422.x"xsd:string
http://purl.uniprot.org/citations/11318877http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-2443.2001.00422.x"xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Goto T."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Goto T."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Yano M."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Yano M."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Nakayama R."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Nakayama R."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Oka C."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Oka C."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Kawaichi M."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Kawaichi M."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Mimura H."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Mimura H."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Mibu Y."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Mibu Y."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Obata J."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/author"Obata J."xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/name"Genes Cells"xsd:string
http://purl.uniprot.org/citations/11318877http://purl.uniprot.org/core/name"Genes Cells"xsd:string
▶