http://purl.uniprot.org/citations/11323029 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/11323029 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/11323029 | http://www.w3.org/2000/01/rdf-schema#comment | "The gene encoding the C-terminal part of A1-domain of human blood coagulation factor VIII (FVIII), a 110-amino acid fragment from Ala(227) to Arg(336), namely A1(Delta1-226), was cloned and used as a 'bait' to screen a protein, which might interact with this region by using the yeast two-hybrid system. A gene coding for a related protein of FVIII named calcium- and integrin-binding protein (CIBP) was isolated from the normal human liver cDNA library. The results were confirmed by using the mammalian two-hybrid system and coimmunoprecipitation. The gene coding for CIBP was constructed by polymerase chain reaction (PCR) and then cotransfected with the B-domain-deleted FVIII gene into mammalian cell lines using the expression vector of FVIII for transient or stable expression. The culture supernatant was collected and analyzed both by enzyme-linked immunosorbent assay (ELISA) for FVIII antigen level and by one-stage method for procoagulant activity. Coexpressed with CIBP, the antigen level of FVIII in the mammalian cell line baby hamster kidney (BHK) cells increased up to about 170% and its bioactivity rose accordingly."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0049-3848(01)00229-8"xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0049-3848(01)00229-8"xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/author | "Chen C."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/author | "Chen C."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/author | "Gu J."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/author | "Gu J."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/author | "Wang Q."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/author | "Wang Q."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/author | "Fang X."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/author | "Fang X."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/author | "Chi C."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/author | "Chi C."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/name | "Thromb. Res."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/name | "Thromb. Res."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/pages | "177-185"xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/pages | "177-185"xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/title | "The interaction of the calcium- and integrin-binding protein (CIBP) with the coagulation factor VIII."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/title | "The interaction of the calcium- and integrin-binding protein (CIBP) with the coagulation factor VIII."xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/volume | "102"xsd:string |
http://purl.uniprot.org/citations/11323029 | http://purl.uniprot.org/core/volume | "102"xsd:string |