| http://purl.uniprot.org/citations/11330669 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11330669 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11330669 | http://www.w3.org/2000/01/rdf-schema#comment | "cDNA clones encoding a major house dust mite allergen, Der f 1, were isolated from a Dermatophagoides farinae cDNA library by plaque immunoscreening using rabbit anti-Der f 1 serum. The sequences cover the complete open reading frame encoding the prepro-form. The sequence is different from previously reported cDNA of Der f 1 in six bases and the encoded amino acid sequence is different in two residues. Pro-forms of Der f 1 and its mutant, in which the N-glycosylation motif was disrupted, expressed in Pichia pastoris were converted to the mature forms by an in vitro activation process and they showed significant IgE-binding. The biologically active rDer f 1 molecules would be useful for diagnostic testing and allergen-specific immunotherapy. In contrast, Der f 1 directly expressed in Escherichia coli without the prosequence had very low IgE binding. The hypoallergenic Der f 1 polypeptide could be useful for safer and more effective immunotherapy."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.org/dc/terms/identifier | "doi:10.1271/bbb.65.563"xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.org/dc/terms/identifier | "doi:10.1271/bbb.65.563"xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/author | "Yuuki T."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/author | "Yuuki T."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/author | "Takai T."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/author | "Takai T."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/author | "Yasuhara T."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/author | "Yasuhara T."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/author | "Okudaira H."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/author | "Okudaira H."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/author | "Okumura Y."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/author | "Okumura Y."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/name | "Biosci. Biotechnol. Biochem."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/name | "Biosci Biotechnol Biochem"xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/pages | "563-569"xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/pages | "563-569"xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/title | "Cloning and expression of cDNA encoding the complete prepro-form of an isoform of Der f 1, the major group 1 allergen from house dust mite Dermatophagoides farinae."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/title | "Cloning and expression of cDNA encoding the complete prepro-form of an isoform of Der f 1, the major group 1 allergen from house dust mite Dermatophagoides farinae."xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/volume | "65"xsd:string |
| http://purl.uniprot.org/citations/11330669 | http://purl.uniprot.org/core/volume | "65"xsd:string |