| http://purl.uniprot.org/citations/11331779 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11331779 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11331779 | http://www.w3.org/2000/01/rdf-schema#comment | "Vsx-1 is a paired-like:CVC homeobox gene whose expression is linked to bipolar cell differentiation during zebrafish retinogenesis. We used a yeast two-hybrid screen to identify proteins interacting with Vsx-1 and isolated Ubc9, an enzyme that conjugates the small ubiquitin-like modifier SUMO-1. Despite its interaction with Ubc9, we show that Vsx-1 is not a substrate for SUMO-1 in COS-7 cells or in vitro. When a yeast two-hybrid assay is used, deletion analysis of the interacting domain on Vsx-1 shows that Ubc9 binds to a nuclear localization signal (NLS) at the NH(2) terminus of the homeodomain. In SW13 cells, Vsx-1 localizes to the nucleus and is excluded from nucleoli. Deletion of the NLS disrupts this nuclear localization, resulting in a diffuse cytoplasmic distribution of Vsx-1. In SW13 AK1 cells that express low levels of endogenous Ubc9, Vsx-1 accumulates in a perinuclear ring and colocalizes with an endoplasmic reticulum marker. However, NLS-tagged STAT1 protein exhibits normal nuclear localization in both SW13 and SW13 AK1 cells, suggesting that nuclear import is not globally disrupted. Cotransfection of Vsx-1 with Ubc9 restores Vsx-1 nuclear localization in SW3 AK1 cells and demonstrates that Ubc9 is required for the nuclear localization of Vsx-1. Ubc9 continues to restore nuclear localization even after a C93S active site mutation has eliminated its SUMO-1-conjugating ability. These results suggest that Ubc9 mediates the nuclear localization of Vsx-1, and possibly other proteins, through a nonenzymatic mechanism that is independent of SUMO-1 conjugation."xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.101129698"xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.101129698"xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/author | "Schechter N."xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/author | "Schechter N."xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/author | "Kurtzman A.L."xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/author | "Kurtzman A.L."xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/pages | "5602-5607"xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/pages | "5602-5607"xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/title | "Ubc9 interacts with a nuclear localization signal and mediates nuclear localization of the paired-like homeobox protein Vsx-1 independent of SUMO-1 modification."xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/title | "Ubc9 interacts with a nuclear localization signal and mediates nuclear localization of the paired-like homeobox protein Vsx-1 independent of SUMO-1 modification."xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/volume | "98"xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://purl.uniprot.org/core/volume | "98"xsd:string |
| http://purl.uniprot.org/citations/11331779 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11331779 |
| http://purl.uniprot.org/citations/11331779 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11331779 |
| http://purl.uniprot.org/citations/11331779 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11331779 |
| http://purl.uniprot.org/citations/11331779 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11331779 |
| http://purl.uniprot.org/uniprot/A0A9U5G231 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/11331779 |
| http://purl.uniprot.org/uniprot/Q9W6H5 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/11331779 |