| http://purl.uniprot.org/citations/11336659 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11336659 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11336659 | http://www.w3.org/2000/01/rdf-schema#comment | "To identify novel protein phosphatase 1 (PP1)-interacting proteins, a yeast two-hybrid 3T3-L1 adipocyte cDNA library was screened with the catalytic subunit of PP1 as bait. In the present work, the isolation, identification and initial biochemical characterization of a novel PP1-interacting protein, MYPT3, which is homologous with the myosin phosphatase targetting subunit (MYPT) family, is described. MYPT3 aligns >99% with a region of mouse genomic DNA clone RP23-156P23 and localizes to chromosome 15, between markers at 44.1-46.5 cM, as demonstrated by radiation hybrid mapping. The gene consists of ten exons that encode for a 524-amino acid sequence with a predicted molecular mass of 57529 Da. The N-terminal region of MYPT3 consists of a consensus PP1-binding site and multiple ankyrin repeats. MYPT3 is distinguished from related approximately 110-130 kDa MYPT subunits by its molecular mass of 58 kDa, and a unique C-terminal region that contains several potential signalling motifs and a CaaX prenylation site. We have shown that affinity-purified glutathione S-transferase (GST)-MYPT3 is prenylated by purified recombinant farnesyltransferase in vitro. Endogenous PP1 from 3T3-L1 lysates specifically interacts with MYPT3. Additionally, purified PP1 activity was inhibited by GST-MYPT3 toward phosphorylase a, myosin light chain and myosin substrate in vitro. Overall, our findings identify a novel prenylatable subunit of PP1 that defines a new subfamily of MYPT."xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.org/dc/terms/identifier | "doi:10.1042/0264-6021:3560257"xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.org/dc/terms/identifier | "doi:10.1042/0264-6021:3560257"xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/author | "Saltiel A.R."xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/author | "Saltiel A.R."xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/author | "Skinner J.A."xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/author | "Skinner J.A."xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/pages | "257-267"xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/pages | "257-267"xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/title | "Cloning and identification of MYPT3: a prenylatable myosin targeting subunit of protein phosphatase 1."xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/title | "Cloning and identification of MYPT3: a prenylatable myosin targeting subunit of protein phosphatase 1."xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/volume | "356"xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://purl.uniprot.org/core/volume | "356"xsd:string |
| http://purl.uniprot.org/citations/11336659 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11336659 |
| http://purl.uniprot.org/citations/11336659 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11336659 |
| http://purl.uniprot.org/citations/11336659 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11336659 |
| http://purl.uniprot.org/citations/11336659 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11336659 |
| http://purl.uniprot.org/uniprot/Q923M0 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/11336659 |
| http://purl.uniprot.org/uniprot/Q923M0#attribution-076BA46712A657BF2C6C1AB9455392B0 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11336659 |