| http://purl.uniprot.org/citations/11342098 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11342098 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11342098 | http://www.w3.org/2000/01/rdf-schema#comment | "To identify regulatory cis-elements in the proximal promoter of the yeast ERG9 squalene synthase gene, promoter deletion analysis was performed. This approach identified two regulatory elements, one an upstream repressing cis-element (URS), and the other an upstream activating cis-element (UAS). Electromobility shift assays (EMSAs) demonstrated that distinct proteins bind each element. Genetic screens were performed to identify yeast mutants that altered expression of ERG9 promoter-reporter gene fusions. Three non-ergosterol biosynthetic pathway genes were identified. A mutation in TPO1(YLL028W) led to a 5.5-fold increase in ERG9 expression while mutations in YER064C and SLK19 (YOR195W) led to a 3.1- and 5.6-fold decrease, respectively. Deletion analysis of these genes demonstrated that TPO1 and SLK19 specifically regulated ERG9 expression when tested with several different promoter-reporter gene fusions. Additionally, EMSAs demonstrated that extracts derived from the TPO1 deletion strain was unable to shift the repressing cis-element while protein extracts from the SLK19 deletion strain had a reduced shift of the activating cis-element. Furthermore, these two mutants showed quantitative differences in sterols and antifungal drug susceptibilities consistent with their role in regulating ERG9 expression."xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0167-4781(00)00246-3"xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0167-4781(00)00246-3"xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/author | "Kennedy M.A."xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/author | "Kennedy M.A."xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/author | "Bard M."xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/author | "Bard M."xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/pages | "177-189"xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/pages | "177-189"xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/title | "Positive and negative regulation of squalene synthase (ERG9), an ergosterol biosynthetic gene, in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/title | "Positive and negative regulation of squalene synthase (ERG9), an ergosterol biosynthetic gene, in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/volume | "1517"xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://purl.uniprot.org/core/volume | "1517"xsd:string |
| http://purl.uniprot.org/citations/11342098 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11342098 |
| http://purl.uniprot.org/citations/11342098 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11342098 |
| http://purl.uniprot.org/citations/11342098 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11342098 |
| http://purl.uniprot.org/citations/11342098 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11342098 |
| http://purl.uniprot.org/uniprot/P40041 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/11342098 |
| http://purl.uniprot.org/uniprot/P40041#attribution-34C7610C76719347336CA0EBDF7C465B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11342098 |