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| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/11350031 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11350031 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11350031 | http://www.w3.org/2000/01/rdf-schema#comment | "prp12-1 is one of the mutants defective in pre-mRNA splicing at a nonpermissive temperature in Schizosaccharomyces pombe. We found that the prp12+ gene encodes a protein highly homologous with a human splicing factor, SAP130/SF3b130, a subunit of a U2 snRNP-associated complex SF3b. Prp12p was shown to interact genetically with Prp10p that is a homolog of SAP155/SF3b155, another subunit in SF3b, suggesting that Prp12p is a functional homolog of human SAP130/SF3b130. Prp12p tagged with GFP is uniformly localized in the nuclear DNA region. In addition to pre-mRNA splicing defects, the prp12-1 mutant produced elongated cells, a typical phenotype of cell division cycle (cdc) mutants, suggesting a possible link between pre-mRNA splicing and cell-cycle progression. We examined kinetics of splicing defects in prp12-1 and several other prp mutants using northern blot hybridization and found that, among all the tested pre-mRNAs, only Tflld pre-mRNA with low splicing efficiency showed detectable splicing defects at the nonpermissive temperature in prp12-1. In addition, we found that other prp mutants with the cdc phenotype also showed differential splicing defects in tested pre-mRNAs at the nonpermissive temperature. On the other hand, prp mutants that do not exhibit the cdc phenotype showed a rapid and complete block of pre-mRNA splicing in all the tested pre-mRNAs at the nonpermissive temperature, indicating that prp mutants with weak splicing defects have a tendency to exhibit the cdc phenotype. These results suggest that the cdc phenotype in prp12-1 is caused by a selective reduction of spliced transcripts encoding a protein (or proteins) required for G2/M transition."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.org/dc/terms/identifier | "doi:10.1017/s1355838201001200"xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.org/dc/terms/identifier | "doi:10.1017/s1355838201001200"xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/author | "Tani T."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/author | "Tani T."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/author | "Shibuya T."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/author | "Shibuya T."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/author | "Ohshima Y."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/author | "Ohshima Y."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/author | "Urushiyama S."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/author | "Urushiyama S."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/author | "Habara Y."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/author | "Habara Y."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/name | "RNA"xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/name | "RNA"xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/pages | "671-681"xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/pages | "671-681"xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/title | "Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/title | "Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe."xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/11350031 | http://purl.uniprot.org/core/volume | "7"xsd:string |