| http://purl.uniprot.org/citations/11350925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11350925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11350925 | http://www.w3.org/2000/01/rdf-schema#comment | "The cyclin-dependent kinase inhibitor p21WAF1/CIP1 is a key regulator of cell-cycle progression and its expression is tightly regulated at the level of transcription and by proteasome-dependent proteolysis. The turnover of p21WAF1/CIP1 by proteasomes does not always require the ubiquitylation of p21WAF1/CIP1 suggesting that there could be an alternative pathway into the proteasome. Here we show that the C8 alpha-subunit of the 20S proteasome interacts with the C-terminus of p21WAF1/CIP1 and mediates the degradation of p21WAF1/CIP1. A small deletion in this region that disrupts binding to C8 increased the half-life of p21WAF1/CIP1 expressed in vivo. In contrast a deletion that increased the affinity between C8 and p21WAF1/CIP1 significantly reduced the stability of the latter. These data suggest that interaction with a 20S proteasome alpha-subunit is a critical determinant of p21WAF1/CIP1 turn-over and show how non-ubiquitylated molecules might bypass the 19S regulator of the proteasome and become targeted directly to the 20S, core protease. Consistent with this, p21WAF1/CIP1 was degraded rapidly by purified 20S proteasomes in a manner that was dependent on the C8-interaction domain."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/20.10.2367"xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/20.10.2367"xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Nakanishi M."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Nakanishi M."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Richardson J."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Richardson J."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Allday M.J."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Allday M.J."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Touitou R."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Touitou R."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Bose S."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Bose S."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Rivett J."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/author | "Rivett J."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/pages | "2367-2375"xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/pages | "2367-2375"xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/title | "A degradation signal located in the C-terminus of p21WAF1/CIP1 is a binding site for the C8 alpha-subunit of the 20S proteasome."xsd:string |
| http://purl.uniprot.org/citations/11350925 | http://purl.uniprot.org/core/title | "A degradation signal located in the C-terminus of p21WAF1/CIP1 is a binding site for the C8 alpha-subunit of the 20S proteasome."xsd:string |