| http://purl.uniprot.org/citations/11356847 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11356847 | http://www.w3.org/2000/01/rdf-schema#comment | "Nun protein from coliphage HK022 binds to phage boxB RNA and functions, in contrast to phage lambda N protein, as a transcriptional terminator. The basic Nun-(10-44) peptide contains the boxB RNA binding arginine rich motif, ARM. The peptide binds boxB RNA and competes with the phage lambda ARM peptide N-(1-36) as indicated by nuclear magnetic resonance (NMR) spectroscopy titrations. In two-dimensional nuclear Overhauser enhancement spectroscopy experiments boxB RNA in complex with Nun-(20-44) exhibits the same pattern of resonances as it does in complex with N peptides containing the ARM, and we could show that Nun-(20-44) forms a bent alpha-helix upon binding to the boxB RNA. The structure of the boxB RNA-bound Nun-(20-44) was determined on the basis of 191 intra- and 30 intermolecular distance restraints. Ser-24 is anchored to the lower RNA stem, and stacking of Tyr-39 and A7 is clearly experimentally indicated. Arg-28 shows numerous contacts to the RNA stem. Leu-22, Ile-30, Trp-33, Ile-37, and Leu-41 form a hydrophobic surface, which could be a recognition site for additional host factors such as NusG. Such a hydrophobic surface area is not present in N-(1-36) bound to boxB RNA."xsd:string |
| http://purl.uniprot.org/citations/11356847 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m102975200"xsd:string |
| http://purl.uniprot.org/citations/11356847 | http://purl.uniprot.org/core/author | "Sticht H."xsd:string |
| http://purl.uniprot.org/citations/11356847 | http://purl.uniprot.org/core/author | "Rosch P."xsd:string |
| http://purl.uniprot.org/citations/11356847 | http://purl.uniprot.org/core/author | "Becker T."xsd:string |
| http://purl.uniprot.org/citations/11356847 | http://purl.uniprot.org/core/author | "Faber C."xsd:string |
| http://purl.uniprot.org/citations/11356847 | http://purl.uniprot.org/core/author | "Scharpf M."xsd:string |
| http://purl.uniprot.org/citations/11356847 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11356847 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/11356847 | http://purl.uniprot.org/core/pages | "32064-32070"xsd:string |
| http://purl.uniprot.org/citations/11356847 | http://purl.uniprot.org/core/title | "The structure of the coliphage HK022 Nun protein-lambda-phage boxB RNA complex. Implications for the mechanism of transcription termination."xsd:string |
| http://purl.uniprot.org/citations/11356847 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11356847 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11356847 |
| http://purl.uniprot.org/citations/11356847 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11356847 |
| http://purl.uniprot.org/uniprot/#_P18683-mappedCitation-11356847 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11356847 |
| http://purl.uniprot.org/uniprot/P18683 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11356847 |