| http://purl.uniprot.org/citations/11387228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11387228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11387228 | http://www.w3.org/2000/01/rdf-schema#comment | "Yeast translation initiation factor 3 contains five core subunits (known as TIF32, PRT1, NIP1, TIF34 and TIF35) and a less tightly associated component known as HCR1. We found that a stable subcomplex of His8-PRT1, NIP1 and TIF32 (PN2 subcomplex) could be affinity purified from a strain overexpressing these eIF3 subunits. eIF5, eIF1 and HCR1 co-purified with this subcomplex, but not with distinct His8-PRT1-TIF34-TIF35 (P45) or His8-PRT1-TIF32 (P2) sub complexes. His8-PRT1 and NIP1 did not form a stable binary subcomplex. These results provide in vivo evidence that TIF32 bridges PRT1 and NIP1, and that eIFs 1 and 5 bind to NIP1, in native eIF3. Heat-treated prt1-1 extracts are defective for Met-tRNA(i)Met binding to 40S subunits, and we also observed defective 40S binding of mRNA, eIFs 1 and 5 and eIF3 itself in these extracts. We could rescue 40S binding of Met-tRNA(i)Met and mRNA, and translation of luciferase mRNA, in a prt1-1 extract almost as well with purified PN2 subcomplex as with five-subunit eIF3, whereas the P45 subcomplex was nearly inactive. Thus, several key functions of eIF3 can be carried out by the PRT1-TIF32-NIP1 subcomplex."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/20.11.2954"xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/20.11.2954"xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/author | "Phan L."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/author | "Phan L."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/author | "Hinnebusch A.G."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/author | "Hinnebusch A.G."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/author | "Schoenfeld L.W."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/author | "Schoenfeld L.W."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/author | "Nielsen K.H."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/author | "Nielsen K.H."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/author | "Valasek L."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/author | "Valasek L."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/pages | "2954-2965"xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/pages | "2954-2965"xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/title | "A subcomplex of three eIF3 subunits binds eIF1 and eIF5 and stimulates ribosome binding of mRNA and tRNA(i)Met."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/title | "A subcomplex of three eIF3 subunits binds eIF1 and eIF5 and stimulates ribosome binding of mRNA and tRNA(i)Met."xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/11387228 | http://purl.uniprot.org/core/volume | "20"xsd:string |