| http://purl.uniprot.org/citations/11402026 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11402026 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11402026 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein recognition within the membrane is a crucial process for numerous biological activities. Detection of such interaction is limited because of difficulties that arise from the hydrophobic environment of the membrane. We detected direct hetero-oligomerization of the glycophorin-A (GPA) transmembrane segments in vivo through inhibition of ToxR transcription activator dimer formation. We investigated the amino acids important for hetero-oligomerization within the membrane, using peptide analog segments of the transmembrane domain of glycophorin A. The wild type ([WT]GPA) and alanine mutant ([A]GPA) were able to interfere with and inhibit the proper dimerization of the ToxR-GPA transcription factor. Conversely, a second alanine mutant ([A(2)]GPA), a glycine mutant ([G]GPA), and a scrambled analog ([SC]GPA) were virtually inactive. Binding studies reveal similar membrane partitions for [WT]GPA, [G]GPA, and [SC]GPA, whereas membrane partition of [A]GPA and [A(2)]GPA are lower. Spectral analysis of fluorescent-labeled analogs revealed a significant blue shift, indicating membrane insertion. Our results suggest that the GXXXG motif, found in homo-oligomerization, is not sufficient for hetero-oligomerization in a biological membrane, whereas an extended motif, LIXXGXXXGXXXT, is sufficient. Interfering with hetero-oligomerization within the membrane can be a useful strategy for characterizing such interactions and possibly modulating membrane protein activity."xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m101889200"xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m101889200"xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/author | "Shai Y."xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/author | "Shai Y."xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/author | "Gerber D."xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/author | "Gerber D."xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/pages | "31229-31232"xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/pages | "31229-31232"xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/title | "In vivo detection of hetero-association of glycophorin-A and its mutants within the membrane."xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/title | "In vivo detection of hetero-association of glycophorin-A and its mutants within the membrane."xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11402026 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11402026 |
| http://purl.uniprot.org/citations/11402026 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11402026 |
| http://purl.uniprot.org/citations/11402026 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11402026 |
| http://purl.uniprot.org/citations/11402026 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11402026 |
| http://purl.uniprot.org/uniprot/P02724 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/11402026 |
| http://purl.uniprot.org/uniprot/P02724#attribution-D0AC2F742C09429EB51FEE7F93AE6394 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11402026 |