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http://purl.uniprot.org/citations/11409545http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11409545http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/11409545http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Citation
http://purl.uniprot.org/citations/11409545http://www.w3.org/2000/01/rdf-schema#comment"Two proteins, component I (CI) and component II (CII), catalyze the biotransformation of crotonobetaine to L(-)-carnitine in Proteus sp. CI was purified to electrophoretic homogeneity from cell-free extracts of Proteus sp. The N-terminal amino acid sequence of CI showed high similarity (80%) to the caiB gene product from Escherichia coli O44K74, which encodes the L(-)-carnitine dehydratase. CI alone was unable to convert crotonobetaine into L(-)-carnitine even in the presence of the cosubstrates crotonobetainyl-CoA or gamma-butyrobetainyl-CoA, which are essential for this biotransformation. The relative molecular mass of CI was determined to be 91.1 kDa. CI is composed of two identical subunits of molecular mass 43.6 kDa. The isoelectric point is 5.0. CII was purified to electrophoretic homogeneity from cell-free extracts of Proteus sp. and its N-terminal amino acid sequence showed high similarity (75%) to the caiD gene product of E. coli O44K74. The relative molecular mass of CII was shown to be 88.0 kDa, and CII is composed of three identical subunits of molecular mass 30.1 kDa. The isoelectric point of CII is 4.9. For the biotransformation of crotonobetaine to L(-)-carnitine, the presence of CI, CII, and a cosubstrate (crotonobetainyl-CoA or gamma-butyrobetainyl-CoA) were shown to be essential."xsd:string
http://purl.uniprot.org/citations/11409545http://purl.org/dc/terms/identifier"doi:10.1007/s002030100272"xsd:string
http://purl.uniprot.org/citations/11409545http://purl.org/dc/terms/identifier"doi:10.1007/s002030100272"xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/author"Elssner T."xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/author"Elssner T."xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/author"Kleber H.-P."xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/author"Kleber H.-P."xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/author"Engemann C."xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/author"Engemann C."xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/date"2001"xsd:gYear
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/name"Arch. Microbiol."xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/name"Arch. Microbiol."xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/pages"353-359"xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/pages"353-359"xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/title"Biotransformation of crotonobetaine to L(-)-carnitine in Proteus sp."xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/title"Biotransformation of crotonobetaine to L(-)-carnitine in Proteus sp."xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/volume"175"xsd:string
http://purl.uniprot.org/citations/11409545http://purl.uniprot.org/core/volume"175"xsd:string
http://purl.uniprot.org/citations/11409545http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/11409545
http://purl.uniprot.org/citations/11409545http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/11409545
http://purl.uniprot.org/citations/11409545http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/11409545