| http://purl.uniprot.org/citations/11447255 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11447255 | http://www.w3.org/2000/01/rdf-schema#comment | "Caenorhabditis elegans is an ideal organism for the study of the molecular basis of fundamental biological processes such as germ-line development, especially because of availability of the whole genome sequence and applicability of the RNA interference (RNAi) technique. To identify genes involved in germ-line development, we produced subtracted cDNA pools either enriched for or deprived of the cDNAs from germ-line tissues. We then performed differential hybridization on the high-density cDNA grid, on which about 7,600 nonoverlapping expressed sequence tag (EST) clones were spotted, to identify a set of genes specifically expressed in the germ line. One hundred and sixty-eight clones were then tested with the RNAi technique. Of these, 15 clones showed sterility with a variety of defects in germ-line development. Seven of them led to the production of unfertilized eggs, because of defects in spermatogenesis (4 clones), or defects in the oocytes (3 clones). The other 8 clones led to failure of oogenesis. These failures were caused by germ-line proliferation defect (Glp phenotype), meiotic arrest, and defects in sperm--oocyte switch (Mog phenotype) among others. These results demonstrate the efficacy of the screening strategy using the EST library combined with the RNAi technique in C. elegans."xsd:string |
| http://purl.uniprot.org/citations/11447255 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.141004698"xsd:string |
| http://purl.uniprot.org/citations/11447255 | http://purl.uniprot.org/core/author | "Kohara Y."xsd:string |
| http://purl.uniprot.org/citations/11447255 | http://purl.uniprot.org/core/author | "Ueno N."xsd:string |
| http://purl.uniprot.org/citations/11447255 | http://purl.uniprot.org/core/author | "Iino Y."xsd:string |
| http://purl.uniprot.org/citations/11447255 | http://purl.uniprot.org/core/author | "Hanazawa M."xsd:string |
| http://purl.uniprot.org/citations/11447255 | http://purl.uniprot.org/core/author | "Mochii M."xsd:string |
| http://purl.uniprot.org/citations/11447255 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11447255 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/11447255 | http://purl.uniprot.org/core/pages | "8686-8691"xsd:string |
| http://purl.uniprot.org/citations/11447255 | http://purl.uniprot.org/core/title | "Use of cDNA subtraction and RNA interference screens in combination reveals genes required for germ-line development in Caenorhabditis elegans."xsd:string |
| http://purl.uniprot.org/citations/11447255 | http://purl.uniprot.org/core/volume | "98"xsd:string |
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