| http://purl.uniprot.org/citations/11448123 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11448123 | http://www.w3.org/2000/01/rdf-schema#comment | "Polymorphonuclear neutrophils (PMNs) are only regarded as being involved in the cleavage of exogenous big endothelin-1 (ET-1) to the active peptide. The aims of the present study were to investigate whether PMNs may themselves express mRNA for prepro-ET-1 (pp-ET-1, a long precursor of 212 amino acids) and to determine the capacity of several PMN stimulants to modulate mRNA expression and the release of ET-1 in culture medium. PMNs, isolated from seven healthy adult volunteers, were stimulated with lipopolysaccharide (LPS, 0.25-10 microg/ml), or LPS (1 microg/ml) + phorbol myristate acetate (PMA, 10 ng/ml) or N-formyl-L-methionyl-L-leucyl-L-phenylalanine (f-MLP, 10(-5) M) or tumour necrosis factor-alpha (TNF-alpha, 50 IU/ml). They were found to express pp-ET-1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Enzyme immunoassay (EIA) revealed low levels of ET-1 in the culture supernatants of PMNs stimulated for 3 h with LPS (10 microg/ml) and with LPS + PMA. Control unstimulated PMNs did not express pp-ET-1 mRNA. The local production of ET-1 by PMNs in vivo has significant implications in inflammatory diseases."xsd:string |
| http://purl.uniprot.org/citations/11448123 | http://purl.org/dc/terms/identifier | "doi:10.1006/cyto.2001.0865"xsd:string |
| http://purl.uniprot.org/citations/11448123 | http://purl.uniprot.org/core/author | "Muscettola M."xsd:string |
| http://purl.uniprot.org/citations/11448123 | http://purl.uniprot.org/core/author | "Mencarelli M."xsd:string |
| http://purl.uniprot.org/citations/11448123 | http://purl.uniprot.org/core/author | "Grasso G."xsd:string |
| http://purl.uniprot.org/citations/11448123 | http://purl.uniprot.org/core/author | "Cambiaggi C."xsd:string |
| http://purl.uniprot.org/citations/11448123 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11448123 | http://purl.uniprot.org/core/name | "Cytokine"xsd:string |
| http://purl.uniprot.org/citations/11448123 | http://purl.uniprot.org/core/pages | "230-233"xsd:string |
| http://purl.uniprot.org/citations/11448123 | http://purl.uniprot.org/core/title | "Gene expression of endothelin-1 (ET-1) and release of mature peptide by activated human neutrophils."xsd:string |
| http://purl.uniprot.org/citations/11448123 | http://purl.uniprot.org/core/volume | "14"xsd:string |
| http://purl.uniprot.org/citations/11448123 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11448123 |
| http://purl.uniprot.org/citations/11448123 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11448123 |
| http://purl.uniprot.org/uniprot/#_P05305-mappedCitation-11448123 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11448123 |
| http://purl.uniprot.org/uniprot/#_Q6FH53-mappedCitation-11448123 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11448123 |
| http://purl.uniprot.org/uniprot/P05305 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11448123 |
| http://purl.uniprot.org/uniprot/Q6FH53 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11448123 |