| http://purl.uniprot.org/citations/11495900 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11495900 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11495900 | http://www.w3.org/2000/01/rdf-schema#comment | "The Saccharomyces cerevisiae pheromone, alpha-factor (WHWLQLKPGQPMY), and Ste2p, its G protein-coupled receptor, were studied as a model for peptide ligand-receptor interaction. The affinities and activities of various synthetic position-10 alpha-factor analogs with Ste2p expressing mutations at residues Ser47 and Thr48 were investigated. All mutant receptors were expressed at a similar level in the cytoplasmic membrane, and their efficacies of signal transduction were similar to that of the wild-type receptor. Mutant receptors differed in binding affinity (Kd) and potency (EC50) for gene induction by alpha-factor. One mutant receptor (S47K,T48K) had dramatically reduced affinity and activity for [Lys10]- and [Orn10]alpha-factor, whereas the affinity for Saccharomyces kluyveri alpha-factor (WHWLSFSKGEPMY) was increased over 20-fold compared with that of wild-type receptor. In contrast, the affinity of [Lys10]- and [Orn10]alpha-factor was increased greatly in a S47E,T48E mutant receptor, whereas the binding of the S. kluyveri alpha-factor was abolished. The affinity of [Lys10]- and [Orn10]alpha-factor for the S47E,T48E receptor dropped 4-6-fold in the presence of 1 m NaCl, whereas the affinity of alpha-factor was not affected by this treatment. These results demonstrate that when bound to its receptor the 10th residue (Gln) of the S. cerevisiae alpha-factor is adjacent to Ser47 and Thr48 residues in the receptor and that the 10th residue of alpha-factors from two Saccharomyces species is responsible for the ligand selectivity to their cognate receptors. Based on these data, we have developed a two-dimensional model of alpha-factor binding to its receptor."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m103579200"xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m103579200"xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/author | "Becker J.M."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/author | "Becker J.M."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/author | "Lee B.K."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/author | "Lee B.K."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/author | "Khare S."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/author | "Khare S."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/author | "Naider F."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/author | "Naider F."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/pages | "37950-37961"xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/pages | "37950-37961"xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/title | "Identification of residues of the Saccharomyces cerevisiae G protein-coupled receptor contributing to alpha-factor pheromone binding."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/title | "Identification of residues of the Saccharomyces cerevisiae G protein-coupled receptor contributing to alpha-factor pheromone binding."xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11495900 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11495900 |
| http://purl.uniprot.org/citations/11495900 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11495900 |