| http://purl.uniprot.org/citations/11509653 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11509653 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11509653 | http://www.w3.org/2000/01/rdf-schema#comment | "Recently, the identification of Clnk, a third member of the SLP-76 family of adaptors expressed exclusively in cytokine-stimulated hemopoietic cells, has been reported by us and by others. Like SLP-76 and Blnk, Clnk was shown to act as a positive regulator of immunoreceptor signaling. Interestingly, however, it did not detectably associate with known binding partners of SLP-76, including Vav, Nck, and GADS. In contrast, it became complexed in activated T cells and myeloid cells with an as yet unknown tyrosine-phosphorylated polypeptide of approximately 92 kDa (p92). In order to understand better the function of Clnk, we sought to identify the Clnk-associated p92. Using a yeast two-hybrid screen and cotransfection experiments with Cos-1 cells, evidence was adduced that p92 is HPK-1, a serine/threonine-specific protein kinase expressed in hemopoietic cells. Further studies showed that Clnk and HPK-1 were also associated in hemopoietic cells and that their interaction was augmented by immunoreceptor stimulation. A much weaker association was detected between HPK-1 and SLP-76. Transient transfections in Jurkat T cells revealed that Clnk and HPK-1 cooperated to increase immunoreceptor-mediated activation of the interleukin 2 (IL-2) promoter. Moreover, the ability of Clnk to stimulate IL-2 promoter activity could be blocked by expression of a kinase-defective version of HPK-1. Lastly we found that in spite of the differential ability of Clnk and SLP-76 to bind cellular proteins, Clnk was apt at rescuing immunoreceptor signaling in a Jurkat T-cell variant lacking SLP-76. Taken together, these results show that Clnk physically and functionally interacts with HPK-1 in hemopoietic cells. Moreover, they suggest that Clnk is capable of functionally substituting for SLP-76 in immunoreceptor signaling, albeit by using a distinct set of intracellular effectors."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.21.18.6102-6112.2001"xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.21.18.6102-6112.2001"xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Yu J."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Yu J."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Arnold R."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Arnold R."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Kiefer F."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Kiefer F."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Riou C."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Riou C."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Davidson D."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Davidson D."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Veillette A."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Veillette A."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Julius M."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Julius M."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Minhas R."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Minhas R."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Robson J.D."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/author | "Robson J.D."xsd:string |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11509653 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |