| http://purl.uniprot.org/citations/11517249 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11517249 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11517249 | http://www.w3.org/2000/01/rdf-schema#comment | "Using a yeast two-hybrid method, we searched for amyloid precursor protein (APP)-interacting molecules by screening mouse and human brain libraries. In addition to known interacting proteins containing a phosphotyrosine-interaction-domain (PID)-Fe65, Fe65L, Fe65L2, X11, and mDab1, we identified, as a novel APP-interacting molecule, a PID-containing isoform of mouse JNK-interacting protein-1 (JIP-1b) and its human homolog IB1, the established scaffold proteins for JNK. The APP amino acids Tyr(682), Asn(684), and Tyr(687) in the G(681)YENPTY(687) region were all essential for APP/JIP-1b interaction, but neither Tyr(653) nor Thr(668) was necessary. APP-interacting ability was specific for this additional isoform containing PID and was shared by both human and mouse homologs. JIP-1b expressed by mammalian cells was efficiently precipitated by the cytoplasmic domain of APP in the extreme Gly(681)-Asn(695) domain-dependent manner. Reciprocally, both full-length wild-type and familial Alzheimer's disease mutant APPs were precipitated by PID-containing JIP constructs. Antibodies raised against the N and C termini of JIP-1b coprecipitated JIP-1b and wild-type or mutant APP in non-neuronal and neuronal cells. Moreover, human JNK1beta1 formed a complex with APP in a JIP-1b-dependent manner. Confocal microscopic examination demonstrated that APP and JIP-1b share similar subcellular localization in transfected cells. These data indicate that JIP-1b/IB1 scaffolds APP with JNK, providing a novel insight into the role of the JNK scaffold protein as an interface of APP with intracellular functional molecules."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.21-17-06597.2001"xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.21-17-06597.2001"xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Ito Y."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Ito Y."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Matsuda S."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Matsuda S."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Niikura T."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Niikura T."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Ohno S."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Ohno S."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Yamamoto T."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Yamamoto T."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Hirai S."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Hirai S."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Hiraki T."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Hiraki T."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Homma Y."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Homma Y."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Kawasumi M."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Kawasumi M."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Kita Y."xsd:string |
| http://purl.uniprot.org/citations/11517249 | http://purl.uniprot.org/core/author | "Kita Y."xsd:string |