| http://purl.uniprot.org/citations/11517336 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11517336 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11517336 | http://www.w3.org/2000/01/rdf-schema#comment | "Genotoxic stresses activate intracellular signaling molecules, which lead to growth arrest, DNA repair, and/or apoptosis. Among these molecules are the growth arrest and DNA damage protein 34 (GADD34) and the Src-related protein tyrosine kinase Lyn. Here, we report that these two proteins physically and functionally interact to regulate DNA damage-induced apoptosis. Multiple isolates of GADD34 and the related murine protein MyD116 were identified as binding partners of Lyn in a yeast two-hybrid screen. The specific interaction was confirmed by in vitro association of GADD34 with glutathione S-transferase fusion proteins containing the Src Homology 3 (SH3) domain of Lyn, as well as coimmunoprecipitation of GADD34 and Lyn from mammalian cells. GADD34 was tyrosine-phosphorylated in vivo in a Lyn-dependent manner. Lyn efficiently phosphorylated affinity-purified GADD34 in vitro. Lyn negatively regulated the proapoptotic function of GADD34 in a kinase-dependent manner. Expression of wild-type, but not kinase-inactive, Lyn weakened promotion of apoptosis by GADD34 following treatment with methyl-methanesulfonate or ionizing radiation in HEK293 and HeLa cells. In contrast, pretreatment of cells with the Src-specific tyrosine kinase inhibitor PP1 strengthened promotion of apoptosis by GADD34. We propose that Lyn regulates the proapoptotic function of GADD34 by binding and phosphorylating it."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.191130798"xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.191130798"xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/author | "Grishin A.V."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/author | "Grishin A.V."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/author | "Corey S.J."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/author | "Corey S.J."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/author | "Azhipa O."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/author | "Azhipa O."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/author | "Semenov I."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/author | "Semenov I."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/pages | "10172-10177"xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/pages | "10172-10177"xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/title | "Interaction between growth arrest-DNA damage protein 34 and Src kinase Lyn negatively regulates genotoxic apoptosis."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/title | "Interaction between growth arrest-DNA damage protein 34 and Src kinase Lyn negatively regulates genotoxic apoptosis."xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/volume | "98"xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://purl.uniprot.org/core/volume | "98"xsd:string |
| http://purl.uniprot.org/citations/11517336 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11517336 |
| http://purl.uniprot.org/citations/11517336 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11517336 |