| http://purl.uniprot.org/citations/11517940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11517940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11517940 | http://www.w3.org/2000/01/rdf-schema#comment | "The saline extract of Bauhinia bauhinioides dry seeds was shown to inhibit cruzipain, a cysteine proteinase from Trypanosoma cruzi. The inhibitory activity was assigned to a protein with 164 amino acid residues and molecular mass of 18 034 Da that was purified by chromatography on DEAE-Sephadex, trypsin-Sepharose (removal of trypsin inhibitors), Mono Q and a reversed-phase C4 column. The primary structure is homologous to other plant Kunitz-type inhibitors, but it lacks cysteine residues and therefore the disulfide bridges. No methionine residue was identified by amino acid sequencing. The inhibition of cruzipain fits into a slow-tight binding mechanism with a low dissociation constant (Ki 1.2 nM). The studied Bauhinia protein also inhibits cruzain (Ki 0.3 nM), a C-terminally truncated recombinant species of cruzipain. Cathepsin L, a cysteine proteinase with high homology to cruzipain, is also inhibited (Ki 0.22 nM), but not cathepsin B, papain, bromelain or ficin."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.org/dc/terms/identifier | "doi:10.1515/bc.2001.103"xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.org/dc/terms/identifier | "doi:10.1515/bc.2001.103"xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Carmona A.K."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Carmona A.K."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Cezari M.H."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Cezari M.H."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Oliva M.L.V."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Oliva M.L.V."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Sampaio C.A.M."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Sampaio C.A.M."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Sampaio M.U."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Sampaio M.U."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Santana L.A."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "Santana L.A."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "de Oliveira C."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/author | "de Oliveira C."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/name | "Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/name | "Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/pages | "847-852"xsd:string |
| http://purl.uniprot.org/citations/11517940 | http://purl.uniprot.org/core/pages | "847-852"xsd:string |