| http://purl.uniprot.org/citations/11526314 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11526314 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11526314 | http://www.w3.org/2000/01/rdf-schema#comment | "The previously unknown crystal structure of the C(1) subunit of the carotenoid-binding protein alpha-crustacyanin has been determined using the anomalous scattering available at 1.77 A wavelength to determine the partial structure of the S atoms intrinsic to the native protein. The resulting 'heavy-atom' phases, in conjunction with near-atomic resolution (d(min) = 1.15 A) data, were then used to initiate successful structure determination using a direct-methods approach. This is, to the authors' knowledge, the first time such a small anomalous signal ( approximately 1%) has been used to aid the determination of a macromolecular structure. As well as the structure itself, the methods used during data collection and those used in the elucidation of the sulfur 'heavy-atom' partial structure are described here. As predicted, the C(1) subunit adopts a tertiary structure typical of the lipocalin superfamily: an eight-stranded antiparallel beta-barrel with a repeated +1 topology. The beta-barrel has a calyx shape with the two molecules in the asymmetric unit interacting in such a way that the open ends of each calyx face each other, although they do not form a single elongated pocket. A comparison of this structure with those of other members of the lipocalin superfamily has allowed speculation as to the nature of carotenoid binding by the protein."xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.org/dc/terms/identifier | "doi:10.1107/s0907444901009362"xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.org/dc/terms/identifier | "doi:10.1107/s0907444901009362"xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/author | "Leonard G.A."xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/author | "Leonard G.A."xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/author | "McSweeney S."xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/author | "McSweeney S."xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/author | "Zagalsky P.F."xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/author | "Zagalsky P.F."xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/author | "Gordon E.J."xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/author | "Gordon E.J."xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/name | "Acta Crystallogr. D"xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/name | "Acta Crystallogr. D"xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/pages | "1230-1237"xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/pages | "1230-1237"xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/title | "The C1 subunit of alpha-crustacyanin: the de novo phasing of the crystal structure of a 40 kDa homodimeric protein using the anomalous scattering from S atoms combined with direct methods."xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/title | "The C1 subunit of alpha-crustacyanin: the de novo phasing of the crystal structure of a 40 kDa homodimeric protein using the anomalous scattering from S atoms combined with direct methods."xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/volume | "57"xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://purl.uniprot.org/core/volume | "57"xsd:string |
| http://purl.uniprot.org/citations/11526314 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11526314 |
| http://purl.uniprot.org/citations/11526314 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11526314 |