| http://purl.uniprot.org/citations/11544199 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11544199 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11544199 | http://www.w3.org/2000/01/rdf-schema#comment | "Here we report a novel design of linker primer that allows one to differentially amplify long tracts (average 3.0 kb with size ranges of 1-7 kb) or short DNAs (average 1.5 kb with size ranges of 0.5-3 kb) from a complex mixture. The method allows one to generate cDNA libraries enriched for long transcripts without size selection of insert DNAs. One representative library from newborn kidney includes 70% of clones bearing ATG start codons. A comparable library has been generated from 20 mouse blastocysts, containing only approximately 40 ng of total RNA. This universal PCR amplification scheme can provide a route to isolate very large cDNAs, even if they are expressed at very low levels."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.org/dc/terms/identifier | "doi:10.1101/gr.185501"xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.org/dc/terms/identifier | "doi:10.1101/gr.185501"xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/author | "Ko M.S.H."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/author | "Ko M.S.H."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/author | "Piao Y."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/author | "Piao Y."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/author | "Lim M.K."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/author | "Lim M.K."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/author | "Ko N.T."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/author | "Ko N.T."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/name | "Genome Res."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/name | "Genome Res."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/pages | "1553-1558"xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/pages | "1553-1558"xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/title | "Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/title | "Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method."xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/11544199 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11544199 |
| http://purl.uniprot.org/citations/11544199 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11544199 |