| http://purl.uniprot.org/citations/11545413 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11545413 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11545413 | http://www.w3.org/2000/01/rdf-schema#comment | "Previous studies in Aspergillus fumigatus (Mouyna I., Fontaine T., Vai M., Monod M., Fonzi W. A., Diaquin M., Popolo L., Hartland R. P., Latgé J.-P, J. Biol. Chem. 2000, 275, 14882-14889) have shown that a glucanosyltransferase playing an important role in fungal cell wall biosynthesis is glycosylphosphatidylinositol (GPI) anchored to the membrane. To identify other GPI-anchored proteins putatively involved in cell wall biogenesis, a proteomic analysis has been undertaken in A. fumigatus and the protein data were matched with the yeast genomic data. GPI-anchored proteins of A. fumigatus were released from membrane preparation by an endogenous GPI-phospholipase C, purified by liquid chromatography and separated by two-dimensional electrophoresis. They were characterized by their peptide mass fingerprint through matrix-assisted laser desorption/ionization-time of flight-(MALDI-TOF)-mass spectrometry and by internal amino acid sequencing. Nine GPI-anchored proteins were identified in A. fumigatus. Five of them were homologs of putatively GPI-anchored yeast proteins (Csa1p, Crh1p, Crh2p, Ecm33p, Gas1p) of unknown function but shown by gene disruption analysis to play a role in cell wall morphogenesis. In addition, a comparative study performed with chitin synthase and glucanosyl transferase mutants of A. fumigatus showed that a modification of the growth phenotype seen in these mutants was associated to an alteration of the pattern of GPI-anchored proteins. These results suggest that GPI-anchored proteins identified in this study are involved in A. fumigatus cell wall organization."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.org/dc/terms/identifier | "doi:10.1002/1522-2683(200108)22:13<2812::aid-elps2812>3.0.co;2-q"xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.org/dc/terms/identifier | "doi:10.1002/1522-2683(200108)22:13<2812::aid-elps2812>3.0.co;2-q"xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Magnin T."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Magnin T."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Latge J.-P."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Latge J.-P."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Legrand R."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Legrand R."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Bernard M."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Bernard M."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Diaquin M."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Diaquin M."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Bruneau J.-M."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Bruneau J.-M."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Fudali C."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Fudali C."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Tagat E."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/author | "Tagat E."xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/name | "Electrophoresis"xsd:string |
| http://purl.uniprot.org/citations/11545413 | http://purl.uniprot.org/core/name | "Electrophoresis"xsd:string |