| http://purl.uniprot.org/citations/11553771 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11553771 | http://www.w3.org/2000/01/rdf-schema#comment | "Possible target proteins of chloroplast thioredoxin (Trx) have been investigated in the stroma lysate of spinach chloroplasts. For that purpose, we immobilized a mutant of m-type Trx in which an internal cysteine at the active site was substituted with serine, on cyanogen bromide-activated resin. By using this resin, the target proteins in chloroplast were efficiently acquired when they formed the mixed-disulfide intermediates with the immobilized Trxs. We could acquire Rubisco activase (45 kDa) and 2-Cys-type peroxiredoxin (Prx), which were recently identified as targets of chloroplast Trxs. Glyceraldehyde-3-phosphate dehydrogenase and sedoheputulose 1,7-bisphosphatase, well-known thiol enzymes in the Calvin cycle, also were recognized among the collected proteins, suggesting the method is applicable for our purpose. Furthermore, four proteins were identified from a homology search of the NH(2)-terminal sequence of the acquired proteins: glutamine synthetase, a protein homologous to chloroplast cyclophilin, a homolog of Prx-Q, and the Rubisco small subunit. The Trx susceptibilities of the recombinant cyclophilin and Prx-Q of Arabidopsis thaliana were then examined. The method developed in the present study is thus applicable to investigate the various redox networks via Trxs and the related enzymes in the cell."xsd:string |
| http://purl.uniprot.org/citations/11553771 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.191282098"xsd:string |
| http://purl.uniprot.org/citations/11553771 | http://purl.uniprot.org/core/author | "Motohashi K."xsd:string |
| http://purl.uniprot.org/citations/11553771 | http://purl.uniprot.org/core/author | "Hisabori T."xsd:string |
| http://purl.uniprot.org/citations/11553771 | http://purl.uniprot.org/core/author | "Kondoh A."xsd:string |
| http://purl.uniprot.org/citations/11553771 | http://purl.uniprot.org/core/author | "Stumpp M.T."xsd:string |
| http://purl.uniprot.org/citations/11553771 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11553771 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/11553771 | http://purl.uniprot.org/core/pages | "11224-11229"xsd:string |
| http://purl.uniprot.org/citations/11553771 | http://purl.uniprot.org/core/title | "Comprehensive survey of proteins targeted by chloroplast thioredoxin."xsd:string |
| http://purl.uniprot.org/citations/11553771 | http://purl.uniprot.org/core/volume | "98"xsd:string |
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