| http://purl.uniprot.org/citations/11555295 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11555295 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11555295 | http://www.w3.org/2000/01/rdf-schema#comment | "The Gram-positive eubacterium Bacillus subtilis is well known for its high capacity to secrete proteins into the environment. Even though high-level secretion of proteins is an efficient process, it imposes stress on the cell. The present studies were aimed at the identification of systems required to combat this so-called secretion stress. A two-component regulatory system, named CssR-CssS, was identified, which bears resemblance to the CpxR-CpxA system of Escherichia coli. The results show that the CssR/S system is required for the cell to survive the severe secretion stress caused by a combination of high-level production of the alpha-amylase AmyQ and reduced levels of the extracytoplasmic folding factor PrsA. As shown with a prsA3 mutation, the Css system is required to degrade misfolded exported proteins at the membrane-cell wall interface. This view is supported by the observation that transcription of the htrA gene, encoding a predicted membrane-bound protease of B. subtilis, is strictly controlled by CssS. Notably, CssS represents the first identified sensor for extracytoplasmic protein misfolding in a Gram-positive eubacterium. In conclusion, the results show that quality control systems for extracytoplasmic protein folding are not exclusively present in the periplasm of Gram-negative eubacteria, but also in the Gram-positive cell envelope."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1365-2958.2001.02576.x"xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1365-2958.2001.02576.x"xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Bron S."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Bron S."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Sarvas M."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Sarvas M."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Pragai Z."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Pragai Z."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "van Dijl J.M."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "van Dijl J.M."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Bolhuis A."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Bolhuis A."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Kontinen V.P."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Kontinen V.P."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Hyyrylaeinen H.-L."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Hyyrylaeinen H.-L."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Darmon E."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Darmon E."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Koski P."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Koski P."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Muukkonen L."xsd:string |
| http://purl.uniprot.org/citations/11555295 | http://purl.uniprot.org/core/author | "Muukkonen L."xsd:string |