| http://purl.uniprot.org/citations/11559781 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11559781 | http://www.w3.org/2000/01/rdf-schema#comment | "1. Phospholamban (PLB) is an inhibitor of the sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA). Its presence and/or functional significance in contractility of bladder, a smooth muscle tissue particularly dependent on SR function, is unknown. We investigated this by measuring the effects of carbachol (CCh) on force and [Ca2+]i in bladder from mice in which the PLB gene was ablated (PLB-KO mice). In the PLB-KO bladder, the maximum increases in [Ca2+]i and force were significantly decreased (41.5 and 47.4 % of WT), and the EC50 values increased. 2. Inhibition of SERCA with cyclopiazonic acid (CPA) abolished these differences between WT and PLB-KO bladder, localizing the effects to the SR. 3. To determine whether these effects were specific to PLB, we generated mice with smooth-muscle-specific expression of PLB (PLB-SMOE mice), using the SMP8 alpha-actin promoter. Western blot analysis of PLB-SMOE mice showed approximately an eightfold overexpression of PLB while SERCA was downregulated 12-fold. 4. In PLB-SMOE bladders, in contrast, the response of [Ca2+]i and force to CCh was significantly increased and the EC50 values were decreased. CPA had little affect on the CCh-induced increases in [Ca2+]i and force in PLB-SMOE bladder. 5. These results show that alteration of the PLB:SERCA ratio can significantly modulate smooth muscle [Ca2+]i. Importantly, our data show that PLB can play a major role in modulation of bladder contractility."xsd:string |
| http://purl.uniprot.org/citations/11559781 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1469-7793.2001.00867.x"xsd:string |
| http://purl.uniprot.org/citations/11559781 | http://purl.uniprot.org/core/author | "Kranias E.G."xsd:string |
| http://purl.uniprot.org/citations/11559781 | http://purl.uniprot.org/core/author | "Paul R.J."xsd:string |
| http://purl.uniprot.org/citations/11559781 | http://purl.uniprot.org/core/author | "Sutliff R.L."xsd:string |
| http://purl.uniprot.org/citations/11559781 | http://purl.uniprot.org/core/author | "Nobe K."xsd:string |
| http://purl.uniprot.org/citations/11559781 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11559781 | http://purl.uniprot.org/core/name | "J Physiol"xsd:string |
| http://purl.uniprot.org/citations/11559781 | http://purl.uniprot.org/core/pages | "867-878"xsd:string |
| http://purl.uniprot.org/citations/11559781 | http://purl.uniprot.org/core/title | "Phospholamban regulation of bladder contractility: evidence from gene-altered mouse models."xsd:string |
| http://purl.uniprot.org/citations/11559781 | http://purl.uniprot.org/core/volume | "535"xsd:string |
| http://purl.uniprot.org/citations/11559781 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11559781 |
| http://purl.uniprot.org/citations/11559781 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11559781 |
| http://purl.uniprot.org/uniprot/#_P61014-mappedCitation-11559781 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11559781 |
| http://purl.uniprot.org/uniprot/P61014 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11559781 |