| http://purl.uniprot.org/citations/11564718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11564718 | http://www.w3.org/2000/01/rdf-schema#comment | "GLP-1 (glucagon-like peptide-1) is a potent insulin secretagogue released from L cells in the intestine. The regulation of GLP-1 secretion has been described both in vivo and in vitro in several animal species, but data from human cellular models are lacking. For this purpose, factors and cell-signaling pathways regulating GLP-1 secretion were investigated in the NCI-H716 human intestinal cell line. After differentiation, these cells homogeneously produced 16.8 pmol GLP-1/mg protein with a basal release of 4.2% during a 2-h incubation period. Nutrients, such as palmitic acid, oleic acid, and meat hydrolysate, stimulated GLP-1 secretion in a dose-dependent manner, as did the cholinergic agonist carbachol and the neuromediator gastrin-releasing peptide. Along with stimulating GLP-1 release, gastrin-releasing peptide, like ionomycin, increased intracellular calcium levels. Activators of PKA and PKC were able to increase GLP-1 secretion in NCI-H716 cells. However, neither PKA activators nor meat hydrolysate increased proglucagon mRNA levels. These findings indicate that the NCI-H716 cell line constitutes a unique model to study the cellular mechanism of GLP-1 secretion in humans and suggest potential interspecies divergence in the regulation of proglucagon gene expression in enteroendocrine cells."xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://purl.org/dc/terms/identifier | "doi:10.1210/endo.142.10.8415"xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://purl.uniprot.org/core/author | "Mace K."xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://purl.uniprot.org/core/author | "Darimont C."xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://purl.uniprot.org/core/author | "Gremlich S."xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://purl.uniprot.org/core/author | "Nicolas-Metral V."xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://purl.uniprot.org/core/author | "Reimer R.A."xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://purl.uniprot.org/core/author | "Ruegg U.T."xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11564718 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://purl.uniprot.org/core/pages | "4522-4528"xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://purl.uniprot.org/core/title | "A human cellular model for studying the regulation of glucagon-like peptide-1 secretion."xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://purl.uniprot.org/core/volume | "142"xsd:string |
| http://purl.uniprot.org/citations/11564718 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11564718 |
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