| http://purl.uniprot.org/citations/11564877 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11564877 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11564877 | http://www.w3.org/2000/01/rdf-schema#comment | "Numerous signaling molecules associate with lipid rafts, either constitutively or after engagement of surface receptors. One such molecule, phospholipase Cgamma-1 (PLCgamma1), translocates from the cytosol to lipid rafts during T-cell receptor (TCR) signaling. To investigate the role played by lipid rafts in the activation of this molecule in T cells, an influenza virus hemagglutinin A (HA)-tagged PLCgamma1 was ectopically expressed in Jurkat T cells and targeted to these microdomains by the addition of a dual-acylation signal. Raft-targeted PLCgamma1 was constitutively tyrosine phosphorylated and induced constitutive NF-AT-dependent transcription and interleukin-2 secretion in Jurkat cells. Tyrosine phosphorylation of raft-targeted PLCgamma1 did not require Zap-70 or the interaction with the adapters Lat and Slp-76, molecules that are necessary for TCR signaling. In contrast, the Src family kinase Lck was required. Coexpression in HEK 293T cells of PLCgamma1-HA with Lck or the Tec family kinase Rlk resulted in preferential phosphorylation of raft-targeted PLCgamma1 over wild-type PLCgamma1. These data show that localization of PLCgamma1 in lipid rafts is sufficient for its activation and demonstrate a role for lipid rafts as microdomains that dynamically segregate and integrate PLCgamma1 with other signaling components."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.21.20.6939-6950.2001"xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.21.20.6939-6950.2001"xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Rawat R."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Rawat R."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Noviello C."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Noviello C."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Graham L."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Graham L."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Wange R.L."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Wange R.L."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Bonvini E."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Bonvini E."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "DeBell K.E."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "DeBell K.E."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "DiBaldassarre A."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "DiBaldassarre A."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Miscia S."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Miscia S."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Reischl I."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Reischl I."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Rellahan B.L."xsd:string |
| http://purl.uniprot.org/citations/11564877 | http://purl.uniprot.org/core/author | "Rellahan B.L."xsd:string |