| http://purl.uniprot.org/citations/11572857 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11572857 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11572857 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/11572857 | http://www.w3.org/2000/01/rdf-schema#comment | "N-Acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST) transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 6 of N-acetylgalactosamine 4-sulfate (GalNAc(4SO(4))) in chondroitin sulfate and dermatan sulfate. We have previously purified the enzyme to apparent homogeneity from the squid cartilage. We report here cloning and characterization of human GalNAc4S-6ST. The strategy for identification of human GalNAc4S-6ST consisted of: 1) determination of the amino acid sequences of peptides derived from the purified squid GalNAc4S-6ST, 2) amplification of squid DNA by polymerase chain reaction, and 3) homology search using the amino acid sequence deduced from the squid DNA. The human GalNAc4S-6ST cDNA contains a single open reading frame that predicts a type II transmembrane protein composed of 561 amino acid residues. The recombinant protein expressed from the human GalNAc4S-6ST cDNA transferred sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 6 of the nonreducing terminal and internal GalNAc(4SO(4)) residues contained in chondroitin sulfate A and dermatan sulfate. When a trisaccharide and a pentasaccharide having sulfate groups at position 4 of N-acetylgalactosamine residues were used as acceptors, only nonreducing terminal GalNAc(4SO(4)) residues were sulfated. The nucleotide sequence of the human GalNAc4S-6ST cDNA was nearly identical to the sequence of human B cell recombination activating gene-associated gene."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m104922200"xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m104922200"xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/author | "Ito Y."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/author | "Ito Y."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/author | "Fukuta M."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/author | "Fukuta M."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/author | "Habuchi O."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/author | "Habuchi O."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/author | "Ohtake S."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/author | "Ohtake S."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/pages | "43894-43900"xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/pages | "43894-43900"xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/title | "Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to human B cell recombination activating gene-associated gene."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/title | "Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to human B cell recombination activating gene-associated gene."xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11572857 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11572857 |