http://purl.uniprot.org/citations/11574696 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/11574696 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/11574696 | http://www.w3.org/2000/01/rdf-schema#comment | "A hairpin ribozyme, RzCR2A, directed against position 323 of the hepatitis C virus 5'-untranslated region (HCV 5'-UTR) was used to establish and validate a novel method for the detection of cellular target molecules for hairpin ribozymes, termed C-SPACE (cleavage-specific amplification of cDNA ends). For C-SPACE, HeLa mRNA containing the transcript of interest was subjected to in vitro cleavage by RzCR2A in parallel with a control ribozyme, followed by reverse transcription using a modified SMART cDNA amplification method and cleavage-specific PCR analysis. C-SPACE allowed identification of the RzCR2A target transcript from a mixture containing the entire cellular mRNA while only requiring knowledge of the ribozyme binding sequence for amplification. In a similar approach, C-SPACE was used successfully to identify human 20S proteasome alpha-subunit PSMA7 mRNA as the cellular target RNA of Rz3'X, a ribozyme originally designed to cleave the negative strand HCV 3'-UTR. Rz3'X was found to substantially inhibit HCV internal ribosome entry site (IRES) activity and PSMA7 was subsequently confirmed to be involved in HCV IRES-mediated translation. Thereby, C-SPACE was validated as a powerful tool to rapidly identify unknown target RNAs recognized and cleaved by hairpin ribozymes."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/29.19.e94"xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/29.19.e94"xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/author | "Kruger M."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/author | "Kruger M."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/author | "Wong-Staal F."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/author | "Wong-Staal F."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/author | "Barber J.R."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/author | "Barber J.R."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/author | "Beger C."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/author | "Beger C."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/author | "Welch P.J."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/author | "Welch P.J."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/pages | "E94"xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/pages | "E94"xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/title | "C-SPACE (cleavage-specific amplification of cDNA ends): a novel method of ribozyme-mediated gene identification."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/title | "C-SPACE (cleavage-specific amplification of cDNA ends): a novel method of ribozyme-mediated gene identification."xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/volume | "29"xsd:string |
http://purl.uniprot.org/citations/11574696 | http://purl.uniprot.org/core/volume | "29"xsd:string |