| http://purl.uniprot.org/citations/11590138 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11590138 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11590138 | http://www.w3.org/2000/01/rdf-schema#comment | "The thrombospondin (TSP) family of extracellular glycoproteins consists of five members in vertebrates, TSP1 to -4 and TSP5/cartilage oligomeric matrix protein, and a single member in Drosophila. TSPs are modular multimeric proteins. The C-terminal end of a monomer consists of 3-6 EGF-like modules; seven tandem 23-, 36-, or 38-residue aspartate-rich, Ca(2+)-binding repeats; and an approximately 230-residue C-terminal sequence. The Ca(2+)-binding repeats and C-terminal sequence are spaced almost exactly the same in different TSPs and share many blocks of identical residues. We studied the C-terminal portion of human TSP2 from the third EGF-like module through the end of the protein (E3CaG2). E3CaG2, CaG2 lacking the EGF module, and Ca2 composed of only the Ca(2+)-binding repeats were expressed using recombinant baculoviruses and purified from conditioned media of insect cells. As previously described for intact TSP1, E3CaG2 bound Ca(2+) in a cooperative manner as assessed by equilibrium dialysis, and its circular dichroism spectrum was sensitive to the presence of Ca(2+). Mass spectrometry of the recombinant proteins digested with endoproteinase Asp-N revealed that disulfide pairing of the 18 cysteines in the Ca(2+)-binding repeats and C-terminal sequence is sequential, i.e. a 1-2, 3-4, 5-6, etc., pattern."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m104218200"xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m104218200"xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/author | "Mosher D.F."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/author | "Mosher D.F."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/author | "Annis D.S."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/author | "Annis D.S."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/author | "Hahr A.J."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/author | "Hahr A.J."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/author | "Harms A.C."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/author | "Harms A.C."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/author | "Misenheimer T.M."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/author | "Misenheimer T.M."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/pages | "45882-45887"xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/pages | "45882-45887"xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/title | "Disulfide connectivity of recombinant C-terminal region of human thrombospondin 2."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/title | "Disulfide connectivity of recombinant C-terminal region of human thrombospondin 2."xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/11590138 | http://purl.uniprot.org/core/volume | "276"xsd:string |