| http://purl.uniprot.org/citations/11673261 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11673261 | http://www.w3.org/2000/01/rdf-schema#comment | "It is generally accepted that cyclic nucleotides are key signaling molecules in the control of oocyte meiotic resumption. Given the role of phosphodiesterases (PDEs) in cyclic nucleotide degradation, this study was undertaken to investigate the properties and regulation of PDEs expressed in rat oocytes. Cilostamide-sensitive PDE3 was the major activity detected in denuded oocytes, whereas no PDE3 activity could be detected in cumulus cells. Moreover, comparable levels of PDE3 activity were measured in cumulus-oocyte complexes (COCs) and in denuded oocytes. The oocyte PDE was recovered in the soluble fraction of the homogenate and immunoprecipitated with a specific PDE3A antibody. A significant and transient increase (P < 0.05) in PDE3 activity was measured in the oocytes after 30 min of culture (70 min after isolation) compared with immediately after collection (10 min after isolation). Conversely, no changes in activity were observed when denuded oocytes or cumulus cells were incubated for up to 130 min. Evaluation of oocyte maturation indicated that only 10% of oocytes had resumed meiosis at the peak of the PDE3 activity. A significant increase (P < 0.05) in PDE3 activity was measured in COCs when follicle-enclosed oocytes were cultured in the presence of hCG. Again, this increase preceded oocyte maturation. In conclusion, these data demonstrate that PDE3A is the major PDE form expressed in mammalian oocytes. PDE3A activity increases prior to resumption of meiosis in both spontaneous and gonadotropin-stimulated maturation. These findings strongly support the hypothesis that an increase in oocyte PDE3A activity is one of the intraoocyte mechanisms controlling resumption of meiosis in rat oocytes, at least in vitro."xsd:string |
| http://purl.uniprot.org/citations/11673261 | http://purl.org/dc/terms/identifier | "doi:10.1095/biolreprod65.5.1444"xsd:string |
| http://purl.uniprot.org/citations/11673261 | http://purl.uniprot.org/core/author | "Conti M."xsd:string |
| http://purl.uniprot.org/citations/11673261 | http://purl.uniprot.org/core/author | "Richard F.J."xsd:string |
| http://purl.uniprot.org/citations/11673261 | http://purl.uniprot.org/core/author | "Tsafriri A."xsd:string |
| http://purl.uniprot.org/citations/11673261 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11673261 | http://purl.uniprot.org/core/name | "Biol Reprod"xsd:string |
| http://purl.uniprot.org/citations/11673261 | http://purl.uniprot.org/core/pages | "1444-1451"xsd:string |
| http://purl.uniprot.org/citations/11673261 | http://purl.uniprot.org/core/title | "Role of phosphodiesterase type 3A in rat oocyte maturation."xsd:string |
| http://purl.uniprot.org/citations/11673261 | http://purl.uniprot.org/core/volume | "65"xsd:string |
| http://purl.uniprot.org/citations/11673261 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/11673261 |
| http://purl.uniprot.org/citations/11673261 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/11673261 |
| http://purl.uniprot.org/uniprot/Q62865#attribution-06CE28E97ABC20DBA30CC3B53E2A8916 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11673261 |
| http://purl.uniprot.org/uniprot/Q62865#attribution-9AC3CCBC8973467BF37D94B3CAD3FA32 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/11673261 |
| http://purl.uniprot.org/uniprot/#_Q62865-mappedCitation-11673261 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11673261 |
| http://purl.uniprot.org/uniprot/Q62865 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/11673261 |