| http://purl.uniprot.org/citations/11687589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11687589 | http://www.w3.org/2000/01/rdf-schema#comment | "Flap endonuclease 1 (FEN-1) is a 5'-3' flap exo-/endonuclease that plays an important role in Okazaki fragment maturation, nonhomologous end joining of double-stranded DNA breaks, and long patch base excision repair. Here, we demonstrate that the wild type FEN-1 binds tightly to chromatin in conjunction with proliferating cell nuclear antigen (PCNA) recruitment after MMS treatment, and the nuclease-defective FEN-1 increased the sensitivity of the cells to methylmethane sulfonate (MMS) and to UV light but not to ionizing radiation. In contrast, the cells expressing the nuclease-defective and PCNA binding-defective double mutant FEN-1 exhibited sensitivities similar to those in the cells expressing the wild type FEN-1. MMS treatment caused a prolonged delay of S phase progression and impairment in colony-forming activity of cells expressing nuclease-defective FEN-1. A comet assay demonstrated that DNA repair after MMS or UV treatment was impaired in the cells expressing nuclease-deficient FEN-1 but not in the cells with double-mutated FEN-1. Taken together, these findings suggest that FEN-1 plays an essential role in the DNA repair processes in mammalian cells and that this activity of FEN-1 is PCNA-dependent."xsd:string |
| http://purl.uniprot.org/citations/11687589 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109461200"xsd:string |
| http://purl.uniprot.org/citations/11687589 | http://purl.uniprot.org/core/author | "Nakamura T."xsd:string |
| http://purl.uniprot.org/citations/11687589 | http://purl.uniprot.org/core/author | "Shibata Y."xsd:string |
| http://purl.uniprot.org/citations/11687589 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/11687589 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/11687589 | http://purl.uniprot.org/core/pages | "746-754"xsd:string |
| http://purl.uniprot.org/citations/11687589 | http://purl.uniprot.org/core/title | "Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay."xsd:string |
| http://purl.uniprot.org/citations/11687589 | http://purl.uniprot.org/core/volume | "277"xsd:string |
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| http://purl.uniprot.org/uniprot/#_Q15054-mappedCitation-11687589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11687589 |
| http://purl.uniprot.org/uniprot/#_P49005-mappedCitation-11687589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11687589 |
| http://purl.uniprot.org/uniprot/#_P15927-mappedCitation-11687589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11687589 |
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| http://purl.uniprot.org/uniprot/#_P28340-mappedCitation-11687589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11687589 |
| http://purl.uniprot.org/uniprot/#_P27694-mappedCitation-11687589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11687589 |
| http://purl.uniprot.org/uniprot/#_P27695-mappedCitation-11687589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11687589 |
| http://purl.uniprot.org/uniprot/#_P35244-mappedCitation-11687589 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/11687589 |