| http://purl.uniprot.org/citations/11687876 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11687876 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/11687876 | http://www.w3.org/2000/01/rdf-schema#comment | "We report here the tissue-specific expression and gabapentin-binding properties of calcium channel alpha2delta subunits. Northern blot analysis demonstrated that human alpha2delta-1, -2, and -3 mRNA all had high levels of expression in brain, heart and skeletal muscle. However, the highest expression of human alpha2delta-2 mRNA was found in lung. Human alpha2delta-1, -2, and -3 mRNAs were detected in all portions of brain tested. Western blotting revealed that alpha2delta-2 protein was predominantly expressed in cerebellar cortex (brain) and undetectable in lung. The dissociation between mRNA and protein levels of human alpha2delta-2 in lung suggests possible post-transcriptional regulation. Although mouse alpha2delta-1 proteins exhibited a similar tissue distribution profile as that of human, tissue distribution of mouse alpha2delta-2 and -3 mRNA revealed a different profile. Mouse alpha2delta-3 mRNA was restricted to brain and mouse alpha2delta-2 mRNA was not detectable in lung. Gel electrophoresis under a reduced condition resulted in a mobility shift of both alpha2delta-1 and alpha2delta-2 proteins, suggesting that alpha2 and delta of alpha2delta-2 protein are linked by disulfide bond as are alpha2 and delta of alpha2delta-1. Scatchard plots revealed a single population of gabapentin binding sites for human alpha2delta-2 with the KD value twofold higher than that of porcine alpha2delta-1 (156 +/- 25 nm vs. 72 +/-9 nm). Inhibition of gabapentin binding to alpha2delta-2 by selected amino acids and gabapentin analogs produced a binding profile similar, but not identical to that of alpha2delta-1."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00232-001-0072-7"xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00232-001-0072-7"xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/author | "Kohler W."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/author | "Kohler W."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/author | "Hang J."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/author | "Hang J."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/author | "Gong H.C."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/author | "Gong H.C."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/author | "Su T.-Z."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/author | "Su T.-Z."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/name | "J. Membr. Biol."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/name | "J. Membr. Biol."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/pages | "35-43"xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/pages | "35-43"xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/title | "Tissue-specific expression and gabapentin-binding properties of calcium channel alpha2delta subunit subtypes."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/title | "Tissue-specific expression and gabapentin-binding properties of calcium channel alpha2delta subunit subtypes."xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/volume | "184"xsd:string |
| http://purl.uniprot.org/citations/11687876 | http://purl.uniprot.org/core/volume | "184"xsd:string |